FITC-Labeled Cynomolgus CD20 / MS4A1 Full Length Protein, His Tag (Nanodisc)

分子別名（Synonym）

MS4A1,CD20,MS4A-1

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

FITC-Labeled Cynomolgus CD20 Full Length Protein, His Tag (CD0-CF2H3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Pro 297 (Accession # M4ZHZ6-1 ).

Predicted N-terminus: Met 1

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 35.2 kDa.

Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein (MSP1D1) coat that confers enhanced stability and a narrow particle size distribution.

The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein(MSP1D1) upon removal of the detergent.

偶聯(lián)（Conjugate）

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

標(biāo)記（Labeling）

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>85% as determined by SDS-PAGE.

制劑（Formulation）

Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

運(yùn)輸（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存儲(chǔ)（Storage）

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

The product MUST be stored at -70°C or lower upon receipt;

-70°C for 3 months under sterile conditions.

背景（Background）

B淋巴細(xì)胞抗原CD20也稱為B淋巴細(xì)胞表面抗原B1、白細(xì)胞表面抗原Leu-16、跨膜4結(jié)構(gòu)域亞家族A成員1和MS4A1，是一種活化的糖基化磷蛋白，從B前相（CD45R+、CD117+）開始在所有B細(xì)胞表面表達(dá)，濃度逐漸增加，直到成熟。CD20在B細(xì)胞發(fā)育的所有階段都有表達(dá)，除了第一個(gè)和最后一個(gè)階段；它從晚期pro-B細(xì)胞通過記憶細(xì)胞存在，但不存在于早期pro-B細(xì)胞或漿母細(xì)胞和漿細(xì)胞上。在B細(xì)胞淋巴瘤、毛細(xì)胞白血病、B細(xì)胞慢性淋巴細(xì)胞白血病和黑色素瘤癌癥干細(xì)胞中發(fā)現(xiàn)。該蛋白沒有已知的天然配體，其功能是實(shí)現(xiàn)最佳的B細(xì)胞免疫反應(yīng)，特別是針對(duì)T非依賴性抗原。懷疑它在細(xì)胞膜中充當(dāng)鈣通道。CD20/MS4A1是單克隆抗體（mAb）利妥昔單抗、伊維單抗和托西莫單抗的靶點(diǎn)，這些抗體都是治療所有B細(xì)胞淋巴瘤和白血病的活性藥物。CD20/MS4A1的缺陷是免疫缺陷共同變量5型（CVID5）的原因；也稱為由于CD20缺陷引起的抗體缺乏癥。CVID5是一種原發(fā)性免疫缺陷，其特征是抗體缺乏、低丙種球蛋白血癥、反復(fù)細(xì)菌感染和無法對(duì)抗原產(chǎn)生抗體反應(yīng)。