Human PTK7 / CCK4 Protein, His Tag (MALS verified)

分子別名（Synonym）

PTK7,CCK4,CCK-4,Tyrosine-protein kinase-like 7,Protein-tyrosine kinase 7,Protein-tyrosine kinase 7

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human PTK7, His Tag (PT7-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 31 - Thr 704 (Accession # Q13308-1).

Predicted N-terminus: Ala 31

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 76.5 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

PTK7在肺、肝、胰腺、腎、胎盤和黑素細(xì)胞中高度表達(dá)。甲狀腺、卵巢、腦、心臟和骨骼肌中表達(dá)較弱。也在紅白血病細(xì)胞中表達(dá)。參與Wnt信號通路的失活酪氨酸激酶。非規(guī)范（也稱為Wnt/平面細(xì)胞極性信號）和規(guī)范Wnt信號通路的組成部分。在細(xì)胞粘附、細(xì)胞遷移、細(xì)胞極性、增殖、肌動蛋白細(xì)胞骨架重組和凋亡中起作用。在胚胎發(fā)生、上皮組織組織和血管生成中起作用。