化合物 AG18|T2063|TargetMol

價格	￥241	￥429	￥847
包裝	5mg	10mg	25mg

最小起訂量	5mg
發(fā)貨地	上海
更新日期	2026-05-08

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產(chǎn)品詳情

中文名稱:化合物 AG18	英文名稱:Tyrphostin 23
CAS:118409-57-7	品牌: TargetMol
產(chǎn)地: 美國	保存條件: Powder: -20°C for 3 years \| In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.
純度規(guī)格: 99.70%	產(chǎn)品類別: 抑制劑
貨號: T2063

Product Introduction

Bioactivity

名稱	Tyrphostin 23
描述	Tyrphostin 23 (AG18) inhibits EGFR with IC50 of 35 μM.
細(xì)胞實(shí)驗(yàn)	GH3 cells are plated at 5 × 104 cells/well in media containing 2% charcoal-stripped FCS and various concentrations of ghrelin, desoctanoylated ghrelin and PMA or EGF for 72 hours with the addition of 2 μCi/well [3H]thymidine for a further 6 hours. A time-course of 24 hours, 48 hours and 72 hours is performed for ghrelin stimulation and 72 hours is selected for further experiments. Studies are also performed to investigate the effect of rat ghrelin or desoctanoyl ghrelin-induced proliferation and the effect of U0126, GF109203X, AG 18, wortmannin and H-89 upon ghrelin-induced MAPK stimulation. AG 18 at 10 μM is added 30 min before each treatment. Cells are harvested before counting in the presence of scintillation fluid using a Microbeta 1450 bcounter. Experiments are repeated at least three times.(Only for Reference)
激酶實(shí)驗(yàn)	EGF-Receptor Autophosphorylation: WGA-purified EGF receptor from A431 cells (0.5 μg/assay) is activated with EGF (800 nM) for 20 min at 4 ℃. The reaction is initiated by the addition of Mg(Ac)2 (60 mM), Tris-Mes buffer, pH 7.6 (50 mM), and [32P]ATP (20 pM, 5 μCi/assay). The reaction is conducted at either 4 ℃ or 15 ℃ and terminated by addition of sodium dodecyl sulfate (SDS) sample buffer (10% glycerol, 50 mM Tris, pH 6.8, 5% β-mercaptoethanol, and 3% SDS). The samples are run on a 8% SDS polyacrylamide gel (SDS-PAGE) (prepared from 30% acrylamide and 0.8% bis-(acrylamide) and contained 0.375 M Tris, pH 8.8, 0.1% SDS, 0.05% TEMED, and 0.46% ammonium persulfate). The gel is dried and autoradiography is perfromed with Agfa Curix RP2 X-ray film. The relevant radioactive bands are cut and counted in the Cerenkov mode. The fast phase of autophosphorylation continued for another 10 min. The extent of phosphorylation completed in the first 10 s at 15 ℃ comprises 1/3 of the total autophosphorylation signal and probably reflects the phosphorylation of the first site on the receptor. The 10-s interval is therefore chosen for use in subsequent autophosphorylation experiments.
體內(nèi)活性	AG18以劑量依賴性方式抑制原代星形膠質(zhì)細(xì)胞培養(yǎng)物中[3H]牛磺酸的體積敏感釋放。AG 18以劑量依賴性方式抑制原代星形膠質(zhì)細(xì)胞培養(yǎng)物中[3H]牛磺酸的體積敏感釋放。 AG 18在A431細(xì)胞中抑制EGF誘導(dǎo)的EGFR自體磷酸化,IC50為15 μM。10 μM AG 18抑制EGF誘導(dǎo)的GH3細(xì)胞增殖。10 μM AG 18抑制GH3細(xì)胞中Ghrelin刺激的ERK1/2磷酸化增加。10 μM AG 18轉(zhuǎn)移2倍KCl誘導(dǎo)的收縮,且產(chǎn)生最大的抑制作用。100 μM AG 18抑制A549上皮細(xì)胞中TNF-alpha和TPA刺激的IKK活性。300 μM AG 18劑量依賴性抑制A549上皮細(xì)胞中TPA誘導(dǎo)的ICAM-1表達(dá)刺激。300 μM AG 18作用于A549 上皮細(xì)胞,也抑制TPA刺激的NF-kappaB DNA-蛋白結(jié)合和ICAM-1啟動子活性。
存儲條件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.
溶解度	10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (10.74 mM), Sonication is recommended. Ethanol : 35 mg/mL (188 mM), Sonication is recommended. H2O : < 1 mg/mL (insoluble or slightly soluble) DMSO : 35 mg/mL (188 mM), Sonication is recommended.
關(guān)鍵字	Tyrphostin-23 \| Tyrphostin23 \| Tyrphostin A-23 \| Tyrphostin A 23 \| Tyrphostin 23 \| TX-825 \| TX825 \| RG50810 \| RG 50810 \| Inhibitor \| inhibit \| HER1 \| ErbB-1 \| Epidermal growth factor receptor \| EGFR \| AG-18 \| AG 18
相關(guān)產(chǎn)品	Neratinib \| Lapatinib \| Chalcone \| Gefitinib \| Erlotinib \| Osimertinib \| Erlotinib hydrochloride \| Afatinib \| Lidocaine Hydrochloride hydrate \| Genistein \| Brigatinib \| (S)-Afatinib
相關(guān)庫	抑制劑庫 \| 抗癌活性化合物庫 \| 經(jīng)典已知活性庫 \| 已知活性化合物庫 \| 細(xì)胞因子抑制劑庫 \| 抗胰腺癌化合物庫 \| 激酶抑制劑庫 \| 細(xì)胞凋亡化合物庫 \| 免疫/炎癥分子化合物庫 \| 膜蛋白靶向化合物庫 \| 酪氨酸激酶分子庫 \| 細(xì)胞重編程化合物庫

關(guān)鍵字： Tyrphostin A23|||TX 825|||RG-50810|||AG18|TargetMol

公司簡介

上海陶術(shù)生物科技有限公司為美國Target Molecule Corp. ( Target Mol ) 在上海建立的全資子公司。我們與美國波士頓、德國慕尼黑的同事一起，為北美、歐洲和亞洲從事藥物研發(fā)和生物學(xué)研究的科學(xué)家提供優(yōu)質(zhì)的產(chǎn)品和專業(yè)的服務(wù)。公司下設(shè)篩選事業(yè)部，化學(xué)事業(yè)部，生物事業(yè)部和新材料部。從虛擬篩選到實(shí)體化合物分子供應(yīng)；從商業(yè)化產(chǎn)品銷售到個性化定制合成；從對明確靶點(diǎn)的分子篩選到對明確分子的多靶點(diǎn)篩選，從高通量篩選到化學(xué)結(jié)構(gòu)優(yōu)化，我們都可以滿足您的科研用品及技術(shù)服務(wù)的需求。經(jīng)過在中國市場五年的精心耕耘，我們已成為篩選化合物領(lǐng)域優(yōu)秀的供應(yīng)商，為超過五百家學(xué)校和各類企業(yè)提供了品質(zhì)卓越的小分子化合物和藥物篩

成立日期	2013-04-18 （14年）	注冊資本	566.2651萬人民幣
員工人數(shù)	100-500人	年營業(yè)額	￥ 1億以上
主營行業(yè)	化學(xué)試劑,生物活性小分子	經(jīng)營模式	貿(mào)易,試劑,定制,服務(wù)

TargetMol中國（陶術(shù)生物）

VIP 14年

公司成立：14年
注冊資本：566.2651萬人民幣
企業(yè)類型：有限責(zé)任公司(自然人投資或控股)
主營產(chǎn)品：小分子抑制劑，藥物篩選化合物庫，天然產(chǎn)物，活性分子化合物等
公司地址：上海市閘北區(qū)江場三路28號4樓

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