Star Ribbon Pre-stained Protein Marker (8-180kDa)
產(chǎn)品描述（Product Details）
This product is a mixture of ten highly purified pre-stained proteins ranging from 8 kDa to 180 kDa. The 72 kDa band is orange, 8 kDa band is green, and other bands are blue. It is designed for observing protein separation during SDS-PAGE, verifying western transfer efficiency on membranes, and approximating the size of proteins.
產(chǎn)品特性（Product Features）
1. Three Pre-Stained Color
2. Neat, Bright and Well-distributed Bands
3. More Accurate Molecular Weight Position
儲存Buffer（Storage Buffer）
20 mM Tris-H3PO4 (pH 7.5), 2 mM EDTA, 1.5 % (W/V) SDS, 3 mM DTT, 0.1% (V/V) Proclin300, 15 % (V/V) Glycerol.
運輸和儲存（Shipping and Storage）
The product is shipped with blue ice. Upon receipt, store it immediately at -20°C for long term storage.
This product is stable after storage at:
-20°C for up to three years or 4°C for up to two months.
使用方法（Procedure）
1. Thaw the product at room temperature for a few minutes to dissolve precipitated solids. Do not boil!
2. Mix gently, but thoroughly, to ensure the solution is homogeneous.
3. Load the following volumes of the product on an SDS-PAGE:
– 3-5 μL per well for mini gel
– 5-10 μL per well for large gel
4. Use the same volumes for Western blot. The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0 mm. The loading volume should be doubled for 1.5 mm thick gels.
產(chǎn)品規(guī)格（Product Specification）
注意事項（Notes）
1. This product has been prepared in 1× SDS-PAGE loading buffer and can be used directly without boiling, diluting and adding reducing agent.
2. Longer transfer times or higher transfer voltages may be required for Western blot of large (>100 kDa) proteins.
3. Don’t add SDS to transfer buffer. If SDS must be used, the concentration should not exceed 0.02-0.04%.
4. In low-percentage gels (< 10 %), the low-molecular weight proteins in the ladder may migrate with the dye front.
5. Pre-stained proteins can have different mobilities in various SDS-PAGE-buffer systems. However, they are suitable for approximate molecular weight determination when calibrated against unstained standards in the same system. See the table provided for migration patterns in different electrophoresis conditions.
6. For your safety and health, please wear a lab coat and disposable gloves.
關鍵字： Protein Marker;預染蛋白marker;預染蛋白;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領域提供關鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學等。