化合物 AI-10-49|T6753|TargetMol

價(jià)格	詢(xún)價(jià)
包裝	1removed

最小起訂量	1removed
發(fā)貨地	上海
更新日期	2026-04-02

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產(chǎn)品詳情

中文名稱(chēng):化合物 AI-10-49	英文名稱(chēng):AI-10-49
CAS:1256094-72-0	品牌: TargetMol
產(chǎn)地: 美國(guó)	保存條件: Powder: -20°C for 3 years \| In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.
純度規(guī)格: 98.75%	產(chǎn)品類(lèi)別: 抑制劑
貨號(hào): T001\|T6753

Product Introduction

Bioactivity

名稱(chēng)	AI-10-49
描述	AI-10-49 is a selective inhibitor of the binding of CBFβ-SMMHC to RUNX1 with IC50 of 260 nM.
細(xì)胞實(shí)驗(yàn)	Cell lines are cultured in IMDM supplemented with 10%~20% fetal bovine serum (FBS) according to culture conditions indicated by ATCC and 1% penicillin/streptomycin (Pen/Strep). All cell lines are tested for mycoplasma. Cells are cultured at 300,000 cells per ml in 96 well plates for 24 and 48 hours in DMSO, or different of AI-10-47, AI-410-49, AI-4-57, or AI-4-88); each in duplicate or triplicate. Cell viability is evaluated using DAPI by flow cytometry. Data is analyzed using FlowJo software and Graphpad Prism software.(Only for Reference)
激酶實(shí)驗(yàn)	FRET assays: Cerulean-Runt domain is expressed and purified. Venus-CBFβ-SMMHC is constructed by inserting 6xHis tag and Venus into pET22b vector between NdeI and NcoI sites, and by inserting CBFβ-SMMHC (the CBFβ-SMMHC construct contains 369 amino acids, 1-166 from CBFβ and 166-369 from MYH11 (amino acids 1526-1730)) between the NcoI and BamHI sites. The fusion protein is purified by standard Ni-affinity chromatography with an on column benzonase treatment to remove residual DNA contaminants. Proteins are dialyzed into FRET buffer (25 mM Tris-HCl, pH 7.5, 150 mM KCl, 2 mM MgCl2) prior to use. Protein concentrations are determined by UV absorbance of the Cerulean and Venus at 433 and 513 nm, respectively. Cerulean-Runt domain and Venus-CBFβ-SMMHC were mixed 1:1 to achieve a final concentration of 10 nM in 96 well black COSTAR plates. DMSO solutions of compounds are added to a final DMSO concentration of 5% (v/v) and the plates incubated at room temperature for one hour in the dark. A PHERAstar microplate reader is used to measure fluorescence (excitation at 433 nm and emission measured at 474 and 525 nm). For IC50 determinations, the ratios of the fluorescence intensities at 525 nm and 474 nm are plotted versus the log of compound concentration, and the resulting curve was fit to a sigmoidal curve using Origin7.0. Three independent measurements are performed and their average and deviation are used for IC50 data fitting.
體外活性	AI-10-49對(duì)帶有inv(16)的ME-1細(xì)胞系顯示出特異性的生長(zhǎng)抑制作用。AI-10-49選擇性地與CBFβ-SMMHC結(jié)合，打斷其與RUNX1的結(jié)合，并恢復(fù)RUNX1的轉(zhuǎn)錄活性。[1]
體內(nèi)活性	在轉(zhuǎn)移了Cbfb+/MYH11;Nras+/G12D白血病細(xì)胞的小鼠中，AI-10-49（200 mg/kg，i.p.）可減緩白血病細(xì)胞擴(kuò)散。[1]
存儲(chǔ)條件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.
溶解度	Ethanol : 93 mg/mL (140.8 mM), Sonication is recommended. 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 3.3 mg/mL (5 mM), Sonication is recommended. DMSO : 93 mg/mL (140.8 mM), Sonication is recommended. H2O : < 1 mg/mL (insoluble or slightly soluble)
關(guān)鍵字	RNASynthesis \| RNA Synthesis \| Inhibitor \| inhibit \| DNASynthesis \| DNA Synthesis \| CBFβ-SMMHC \| AI-10-49 \| AI1049 \| AI 10 49
相關(guān)產(chǎn)品	Carbazole \| Hexane-1,6-diol \| Guanidine hydrochloride \| Vidarabine \| 1,4-Naphthoquinone \| 5-Fluorouracil \| Usnic Acid \| Adenine hemisulfate \| Erythromycin thiocyanate \| Adenine \| Docosanoic acid \| Thymidine
相關(guān)庫(kù)	抑制劑庫(kù) \| 經(jīng)典已知活性庫(kù) \| 抗癌化合物庫(kù) \| 已知活性化合物庫(kù) \| 細(xì)胞周期化合物庫(kù) \| 含氟化合物庫(kù) \| 抗衰老化合物庫(kù) \| NO PAINS 化合物庫(kù) \| DNA 損傷和修復(fù)分子庫(kù)

關(guān)鍵字： AI-10-49;TargetMol

公司簡(jiǎn)介

TargetMol Chemicals Inc. 總部位于馬薩諸塞州波士頓，致力于為全球生化領(lǐng)域科學(xué)家的研究提供專(zhuān)業(yè)的產(chǎn)品和服務(wù)。TargetMol?品牌的客戶(hù)群分布于40多個(gè)國(guó)家和地區(qū)，已發(fā)展成為全球知名的化合物庫(kù)和小分子化合物研究供應(yīng)商。 TargetMol?可提供160多種滿(mǎn)足不同需求的化合物庫(kù)，以及多種類(lèi)型的生化試劑產(chǎn)品，包括12000多種抑制劑、16000多種天然產(chǎn)物和各類(lèi)多肽、抗體、生命科學(xué)試劑盒等，此外，我們還建設(shè)有CADD（計(jì)算機(jī)輔助藥物設(shè)計(jì)）研究中心、藥理實(shí)驗(yàn)室、藥化合成平臺(tái)三大技術(shù)中心，全方位滿(mǎn)足客戶(hù)的定制需求。憑借我們優(yōu)質(zhì)的產(chǎn)品和服務(wù)、快速高效的全球供應(yīng)鏈和專(zhuān)業(yè)的技術(shù)支持，我們將有效幫助您縮短研發(fā)周期，取得更成功的結(jié)果。

成立日期	2013-04-18 （14年）	注冊(cè)資本	566.265100萬(wàn)人民幣
員工人數(shù)	100-500人	年?duì)I業(yè)額	￥ 1億以上
主營(yíng)行業(yè)	天然產(chǎn)物,生化試劑,分子生物學(xué),分子砌塊,生物技術(shù)服務(wù)	經(jīng)營(yíng)模式	貿(mào)易,工廠(chǎng),試劑,定制,服務(wù)

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公司成立：14年
注冊(cè)資本：566.265100萬(wàn)人民幣
企業(yè)類(lèi)型：有限責(zé)任公司(自然人投資或控股)
主營(yíng)產(chǎn)品：小分子抑制劑、藥物篩選化合物庫(kù)、藥物篩選等
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