| 中文名稱:RUNX1抗體 | 英文名稱:Mouse Monoclonal RUNX1 Antibody |
| 品牌: 艾普蒂 | 產(chǎn)地: 武漢 |
| 保存條件: Stored at -20°C for 376 year. Avoid repeated freeze / thaw cycles. | 產(chǎn)品類別: 抗體 |
| 重組: 否 | 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA |
| 種屬反應(yīng)性: Human,Mouse,Rat | 宿主: Mouse |
| 偶聯(lián)物: 無 | 靶點(diǎn): RUNX1 |
| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨詢技術(shù) | Human,Mouse,Rat |
| IHC | 咨詢技術(shù) | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 咨詢技術(shù) | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1; RUNX1 |
| Entrez GeneID | 861 |
| clone | 5A1 |
| WB Predicted band size | 55kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide of human RUNX1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Western blot analysis using RUNX1 mouse mAb against Jurkat cell lysate.
Immunofluorescence analysis of Hela cells using RUNX1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
以下是3篇關(guān)于RUNX1抗體的參考文獻(xiàn)及其摘要概括:
1. **文獻(xiàn)名稱**:*"RUNX1 in normal and malignant hematopoiesis: Role in cellular differentiation and disease progression"*
**作者**:Ito Y et al.
**摘要**:該研究探討RUNX1在造血系統(tǒng)中的調(diào)控作用,利用特異性抗體檢測RUNX1在白血病細(xì)胞中的異常表達(dá),揭示其突變導(dǎo)致轉(zhuǎn)錄活性失調(diào)與急性髓系白血病(AML)的關(guān)聯(lián)。
2. **文獻(xiàn)名稱**:*"Antibody-based detection of RUNX1 truncations in therapy-related myeloid neoplasms"*
**作者**:Osato M & Ito Y
**摘要**:通過Western blot和免疫組化方法,驗(yàn)證多種RUNX1抗體的特異性,發(fā)現(xiàn)截短型RUNX1蛋白在放化療后繼發(fā)性血液腫瘤中的高表達(dá),提示其作為診斷標(biāo)志物的潛力。
3. **文獻(xiàn)名稱**:*"ChIP-seq analysis of RUNX1 binding sites in hematopoietic progenitors using a novel monoclonal antibody"*
**作者**:Growney JD et al.
**摘要**:開發(fā)并驗(yàn)證一種高特異性抗RUNX1單克隆抗體,結(jié)合染色質(zhì)免疫沉淀測序(ChIP-seq)技術(shù),繪制造血干細(xì)胞中RUNX1的基因組結(jié)合圖譜,揭示其在調(diào)控關(guān)鍵靶基因(如PU.1)中的功能。
4. **文獻(xiàn)名稱**:*"RUNX1 mutations impair hematopoietic differentiation by disrupting co-factor recruitment"*
**作者**:Sood R et al.
**摘要**:通過免疫共沉淀(Co-IP)結(jié)合RUNX1抗體,發(fā)現(xiàn)白血病相關(guān)突變會破壞RUNX1與CBFβ等輔因子的相互作用,導(dǎo)致造血分化阻滯及惡性轉(zhuǎn)化機(jī)制。
這些研究均依賴不同RUNX1抗體的應(yīng)用,涵蓋疾病機(jī)制、診斷技術(shù)及基因組調(diào)控等領(lǐng)域。
**Background of RUNX1 Antibody**
The RUNX1 (Runt-related transcription factor 1) antibody is a critical tool for studying the RUNX1 protein, a member of the RUNX family of transcription factors that play pivotal roles in hematopoiesis, cellular differentiation, and oncogenesis. RUNX1. also known as AML1 (Acute Myeloid Leukemia 1), binds DNA via its conserved Runt domain and regulates gene expression by forming complexes with co-factors like CBFβ. It is essential for the development of hematopoietic stem cells and megakaryocyte maturation.
Dysregulation or mutations in RUNX1 are closely associated with hematologic malignancies, particularly acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS). Somatic mutations, chromosomal translocations (e.g., RUNX1-ETO in t(8;21) AML), or inherited RUNX1 variants can disrupt its normal function, leading to impaired differentiation and proliferation of blood cells.
RUNX1 antibodies are widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunohistochemistry (IHC), and chromatin immunoprecipitation (ChIP). They also aid in diagnosing RUNX1-related disorders and evaluating therapeutic targets. Specific antibodies may recognize different isoforms (e.g., RUNX1a, b, c) or post-translational modifications, enabling precise investigation of its roles in health and disease. As RUNX1 continues to be a focus of cancer biology and stem cell research, its antibody remains indispensable for both basic and clinical studies.
| 成立日期 | 2024-06-24 (3年) | 注冊資本 | 50萬(元) |
| 員工人數(shù) | 1-10人 | 年?duì)I業(yè)額 | ¥ 100萬以內(nèi) |
| 主營行業(yè) | 中間體,化學(xué)試劑 | 經(jīng)營模式 | 貿(mào)易,工廠,試劑,定制,服務(wù) |
| 產(chǎn)品名稱 | 價(jià)格 | 公司名稱 | 報(bào)價(jià)日期 | |
|---|---|---|---|---|
| ¥1098 |
VIP4年
|
上海滬震實(shí)業(yè)有限公司
|
2026-07-24 | |
| 詢價(jià) |
上海切爾齊生物科技有限公司
|
2025-05-23 | ||
| 詢價(jià) |
碧云天生物技術(shù)有限公司
|
2026-07-12 |