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ABCG2抗體,Mouse Monoclonal ABCG2 Antibody
  • ABCG2抗體,Mouse Monoclonal ABCG2 Antibody
  • ABCG2抗體,Mouse Monoclonal ABCG2 Antibody
  • ABCG2抗體,Mouse Monoclonal ABCG2 Antibody

ABCG2抗體

價格 詢價
包裝 20μl 50μl 100μl
最小起訂量 1μl
發(fā)貨地 湖北
更新日期 2025-05-09
QQ交談 微信洽談

產(chǎn)品詳情

中文名稱:ABCG2抗體英文名稱:Mouse Monoclonal ABCG2 Antibody
品牌: 艾普蒂產(chǎn)地: 武漢
保存條件: Stored at -20°C for 408 year. Avoid repeated freeze / thaw cycles.產(chǎn)品類別: 抗體
重組: 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA
種屬反應(yīng)性: Human,Mouse,Rat 宿主: Mouse
偶聯(lián)物: 靶點: ABCG2
2025-05-09 ABCG2抗體 Mouse Monoclonal ABCG2 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武漢 Stored at -20°C for 408 year. Avoid repeated freeze / thaw cycles. 抗體

驗證與應(yīng)用

應(yīng)用及物種
WB1/500 - 1/2000 Human,Mouse,Rat
IF咨詢技術(shù) Human,Mouse,Rat
IHC1/100 - 1/500 Human,Mouse,Rat
ICC1/50 - 1/500 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

產(chǎn)品詳情

AliasesMRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; CDw338; EST157481; MGC102821
Entrez GeneID9429
clone3G8
WB Predicted band size72kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat,Rabbit,Monkey
ImmunogenPurified recombinant fragment of human ABCG2 expressed in E. Coli.  
FormulationPurified antibody in PBS with 0.05% sodium azide.

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       Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    


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       Western blot analysis using ABCG2 mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.    


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       Immunofluorescence analysis of Hela cells using ABCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    


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       Flow cytometric analysis of HepG2 cells using ABCG2 mouse mAb (green) and negative control (purple).    


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       Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues (left) and skeletal muscle tissues (right) using ABCG2 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Mouse muscle using ABCG2 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Rat muscle using ABCG2 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Rabbit muscle using ABCG2 mouse mAb with DAB staining.    


           

參考文獻

以下是3-4條關(guān)于ABCG2抗體的參考文獻及其摘要內(nèi)容:

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1. **文獻名稱**:*Multidrug resistance mediated by the breast cancer resistance protein (ABCG2)*

**作者**:Doyle, L.A., & Ross, D.D.

**摘要**:該研究首次克隆并表征了ABCG2(BCRP)基因,揭示了其作為ATP結(jié)合盒轉(zhuǎn)運蛋白在腫瘤多藥耐藥中的作用,并開發(fā)了特異性抗體用于檢測其在癌細(xì)胞中的過表達(dá)。

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2. **文獻名稱**:*Subcellular localization and distribution of the breast cancer resistance protein transporter in normal human tissues*

**作者**:Maliepaard, M., et al.

**摘要**:通過免疫組化分析,研究利用ABCG2特異性抗體揭示了該蛋白在胎盤、肝臟、小腸等正常組織中的分布特征,為理解其生理功能及藥物外排機制提供依據(jù)。

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3. **文獻名稱**:*Comparison of anti-ABCG2 monoclonal antibodies for specificity and sensitivity in immunohistochemistry*

**作者**:Mao, Q., & Minderman, H.

**摘要**:研究系統(tǒng)評估了多種商業(yè)ABCG2抗體的特異性和靈敏度,發(fā)現(xiàn)部分抗體存在交叉反應(yīng)性,強調(diào)選擇高特異性抗體對臨床樣本分析的重要性。

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4. **文獻名稱**:*Breast cancer resistance protein (BCRP/ABCG2): its role in multidrug resistance and regulation of gene expression*

**作者**:Nakanishi, T., & Ross, D.D.

**摘要**:綜述總結(jié)了ABCG2在腫瘤耐藥中的分子機制,并討論了抗體在檢測其表達(dá)水平及功能研究中的應(yīng)用,強調(diào)其在個體化治療中的潛在價值。

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以上文獻涵蓋了ABCG2的功能研究、抗體開發(fā)及臨床應(yīng)用,可作為相關(guān)領(lǐng)域的基礎(chǔ)參考。

       

背景信息

ABCG2 (ATP-binding cassette subfamily G member 2), also known as breast cancer resistance protein (BCRP), is a transmembrane transporter protein belonging to the ATP-binding cassette (ABC) superfamily. It plays a critical role in cellular detoxification by effluxing a wide range of endogenous and exogenous substrates, including chemotherapeutic agents, toxins, and metabolic byproducts. ABCG2 is expressed in various tissues, such as the placenta, blood-brain barrier, liver, and intestines, where it contributes to tissue protection and drug pharmacokinetics. Its overexpression in cancer cells is linked to multidrug resistance (MDR), a major challenge in oncology, as it reduces intracellular drug accumulation and therapeutic efficacy.

ABCG2 antibodies are essential tools for studying the protein's expression, localization, and functional roles. These antibodies enable detection via techniques like Western blotting, immunohistochemistry, and flow cytometry, aiding in research on drug resistance mechanisms, stem cell biology (e.g., side population identification), and barrier tissue functions. Monoclonal and polyclonal antibodies targeting specific epitopes of ABCG2 are validated for specificity and sensitivity in diverse experimental models. Recent advances in therapeutic strategies targeting ABCG2. such as inhibitors to overcome MDR or nanoparticle-based drug delivery systems, further underscore the importance of reliable antibodies in both basic research and clinical applications.

       
關(guān)鍵字: ABCG2抗體;ABCG2;ABCG2 Antibody;

公司簡介

湖北瑞和寧生物科技有限公司研究創(chuàng)新能力, 擁有完整、科學(xué)、質(zhì)量管理體系。并不斷致力于技術(shù)創(chuàng)新,產(chǎn)品創(chuàng)新和管理創(chuàng)新,贏得了國內(nèi)外廣大用戶的信任。
成立日期 2024-06-24 (3年) 注冊資本 50萬(元)
員工人數(shù) 1-10人 年營業(yè)額 ¥ 100萬以內(nèi)
主營行業(yè) 中間體,化學(xué)試劑 經(jīng)營模式 貿(mào)易,工廠,試劑,定制,服務(wù)
  • 湖北瑞和寧生物科技有限公司
普通會員
  • 公司成立:3年
  • 注冊資本:50萬(元)
  • 企業(yè)類型:有限責(zé)任公司(自然人投資或控股)
  • 主營產(chǎn)品:中間體。試劑
  • 公司地址:江漢區(qū)
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