| 中文名稱:PRK2抗體 | 英文名稱:Mouse Monoclonal PRK2 Antibody |
| 品牌: 艾普蒂 | 產(chǎn)地: 武漢 |
| 保存條件: Stored at -20°C for 433 year. Avoid repeated freeze / thaw cycles. | 產(chǎn)品類別: 抗體 |
| 重組: 否 | 應用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA |
| 種屬反應性: Human,Mouse,Rat | 宿主: Mouse |
| 偶聯(lián)物: 無 | 靶點: PRK2 |
| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨詢技術(shù) | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技術(shù)咨詢 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PKN2; PAK2; PRK2; Pak-2; PRKCL2; PRO2042; MGC71074; MGC150606 |
| Entrez GeneID | 5586 |
| clone | 1D1 |
| WB Predicted band size | 140kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Purified recombinant fragment of human PRK2 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Western blot analysis using PRK2 mouse mAb against PC-12 (1), Cos7 (2), K562 (3), Jurkat (4), Hela (5), A431 (6), C6 (7), NIH/3T3 (8) and HEK293 (9) cell lysate.
Flow cytometric analysis of NIH/3T3 cells using PRK2 mouse mAb (blue) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human prostate tissues using PRK2 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using PRK2 mouse mAb with DAB staining.
以下是關(guān)于PRK2(Protein Kinase C-related kinase 2)抗體的3篇參考文獻的簡要總結(jié):
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1. **文獻名稱**: "PRK2/PKNγ regulates keratinocyte activation by suppressing EGFR signaling through phosphorylation of EGFR-T654"
**作者**: Takahashi H, et al.
**摘要**: 本研究利用PRK2特異性抗體(Western blot和免疫熒光)發(fā)現(xiàn)PRK2通過磷酸化EGFR-T654抑制其活性,調(diào)控角質(zhì)形成細胞的增殖和分化,揭示了PRK2在皮膚屏障修復中的作用。
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2. **文獻名稱**: "PKN2/PRK2 promotes cell migration and tumor growth via the MEK-ERK pathway"
**作者**: Kim J, et al.
**摘要**: 通過PRK2抗體(免疫組化、siRNA實驗)證明,PRK2在多種癌細胞中高表達,通過激活MEK-ERK信號通路促進腫瘤遷移和生長,提示其作為癌癥治療靶點的潛力。
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3. **文獻名稱**: "PRK2 interacts with Amyloid-β precursor protein and regulates its trafficking"
**作者**: Lee S, et al.
**摘要**: 使用PRK2抗體(免疫共沉淀)發(fā)現(xiàn)PRK2與β淀粉樣前體蛋白(APP)結(jié)合,影響APP的胞內(nèi)運輸和Aβ生成,為阿爾茨海默病機制研究提供了新方向。
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提示:如需具體文獻來源或更早年的研究,可進一步提供領(lǐng)域方向(如癌癥、神經(jīng)科學等)。
**Background of PRK2 Antibody**
The PRK2 (protein kinase C-related kinase 2) antibody is a tool used to detect and study PRK2. a serine/threonine kinase belonging to the AGC family of protein kinases. PRK2. also known as PKN2 or Pkn2. shares structural homology with protein kinase C (PKC) and plays critical roles in diverse cellular processes, including cytoskeletal reorganization, cell migration, proliferation, and apoptosis. It is activated by Rho GTPases (e.g., RhoA, Rac1) and interacts with signaling pathways such as mTOR and PI3K/AKT, making it a key regulator of cellular responses to extracellular stimuli.
PRK2 antibodies are essential for investigating its expression, localization, and activation in various tissues and disease contexts. Dysregulation of PRK2 has been implicated in cancer, neurodegenerative disorders, and cardiovascular diseases. For instance, elevated PRK2 levels correlate with tumor progression and metastasis in certain cancers. Researchers utilize PRK2 antibodies in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to explore its functional mechanisms or diagnostic potential.
These antibodies are often validated for specificity against PRK2 isoforms or phosphorylated forms (e.g., Thr816/Thr822), ensuring accurate detection in experimental models. Their application aids in deciphering PRK2's role in health and disease, supporting drug discovery and biomarker development.
| 成立日期 | 2024-06-24 (3年) | 注冊資本 | 50萬(元) |
| 員工人數(shù) | 1-10人 | 年營業(yè)額 | ¥ 100萬以內(nèi) |
| 主營行業(yè) | 中間體,化學試劑 | 經(jīng)營模式 | 貿(mào)易,工廠,試劑,定制,服務 |
| 產(chǎn)品名稱 | 價格 | 公司名稱 | 報價日期 | |
|---|---|---|---|---|
| 詢價 |
VIP4年
|
維百奧(北京)生物科技有限公司
|
2026-07-30 | |
| ¥1800 |
VIP2年
|
費雪(杭州)醫(yī)學研究有限公司
|
2026-07-27 | |
| 詢價 |
上海華盈生物醫(yī)藥科技有限公司
|
2026-07-28 |