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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Flow cytometric analysis of HepG2 cells using WDR66 mouse mAb (green) and negative control (purple).    

  
  

The WDR66 antibody is a research tool designed to detect the WD repeat-containing protein 66 (WDR66), also known as C2orf40 or CFAP169. WDR66 is a member of the WD-repeat protein family, characterized by conserved structural motifs that facilitate protein-protein interactions and cellular processes. This protein is predominantly expressed in tissues with motile cilia or flagella, such as the testis, respiratory tract, and fallopian tubes. Studies suggest WDR66 plays a role in ciliary/flagellar assembly and motility, particularly in spermatozoa, where it localizes to the fibrous sheath of the sperm tail and may regulate signaling pathways critical for sperm function.

WDR66 antibodies are widely used in molecular biology and reproductive medicine to investigate protein expression, localization, and functional mechanisms. They are essential for techniques like Western blotting, immunohistochemistry, and immunofluorescence. Research involving WDR66 has implications for understanding male infertility, as mutations or dysregulation of WDR66 are linked to sperm motility disorders. Additionally, its potential role in cilia-related pathologies, such as respiratory diseases or developmental defects, is an emerging area of interest.

These antibodies are typically generated in animal hosts (e.g., rabbits or mice) using purified recombinant WDR66 fragments, ensuring specificity. Validation includes testing against knockout cell lines or tissues to confirm target selectivity. Overall, WDR66 antibodies serve as vital reagents for exploring the molecular basis of ciliary function and associated diseases.