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ZEB1抗體,Mouse Monoclonal ZEB1 Antibody
  • ZEB1抗體,Mouse Monoclonal ZEB1 Antibody
  • ZEB1抗體,Mouse Monoclonal ZEB1 Antibody
  • ZEB1抗體,Mouse Monoclonal ZEB1 Antibody

ZEB1抗體

價格 詢價
包裝 20μl 50μl 100μl
最小起訂量 1μl
發(fā)貨地 湖北
更新日期 2025-05-10
QQ交談 微信洽談

產(chǎn)品詳情

中文名稱:ZEB1抗體英文名稱:Mouse Monoclonal ZEB1 Antibody
品牌: 艾普蒂產(chǎn)地: 武漢
保存條件: Stored at -20°C for 612 year. Avoid repeated freeze / thaw cycles.產(chǎn)品類別: 抗體
重組: 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA
種屬反應(yīng)性: Human,Mouse,Rat 宿主: Mouse
偶聯(lián)物: 靶點: ZEB1
2025-05-10 ZEB1抗體 Mouse Monoclonal ZEB1 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武漢 Stored at -20°C for 612 year. Avoid repeated freeze / thaw cycles. 抗體

驗證與應(yīng)用

應(yīng)用及物種
WB咨詢技術(shù) Human,Mouse,Rat
IF咨詢技術(shù) Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

產(chǎn)品詳情

AliasesBZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
Entrez GeneID6935
clone2A8A6
WB Predicted band size124kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human ZEB1 (AA: 967-1108) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


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       Immunofluorescence analysis of Hela cells using ZEB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    


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       Flow cytometric analysis of Hela cells using ZEB1 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using ZEB1 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using ZEB1 mouse mAb with DAB staining.    


           

參考文獻

以下是3篇關(guān)于ZEB1抗體的參考文獻及其摘要概括:

1. **"ZEB1: A Critical Regulator of Cell Plasticity and DNA Damage Response"**

- Authors: Zhang P, Sun Y, Ma L

- 摘要:該研究驗證了兔源ZEB1抗體(貨號AB123)在乳腺癌細(xì)胞系中的特異性,通過免疫共沉淀和Western blot證明其能有效識別ZEB1蛋白,并揭示ZEB1通過調(diào)控EMT促進化療耐藥。

2. **"Antibody Validation for Epithelial-Mesenchymal Transition Markers"**

- Authors: Vandewalle C, Van Roy F

- 摘要:系統(tǒng)性評估多種EMT相關(guān)抗體,包括小鼠單克隆ZEB1抗體(Clone 3F10)。通過免疫熒光和流式細(xì)胞術(shù)確認(rèn)其在結(jié)腸癌細(xì)胞中的特異性,發(fā)現(xiàn)ZEB1高表達(dá)與轉(zhuǎn)移相關(guān)。

3. **"ZEB1 Antibody Application in Pancreatic Cancer Studies"**

- Authors: Singh A, Settleman J

- 摘要:使用兔多克隆ZEB1抗體(貨號HPA027524)分析胰腺癌組織芯片,發(fā)現(xiàn)ZEB1在腫瘤基質(zhì)中的表達(dá)與患者預(yù)后負(fù)相關(guān),抗體經(jīng)肽段競爭實驗驗證特異性。

注:以上為虛構(gòu)示例,實際文獻需通過PubMed/Google Scholar檢索關(guān)鍵詞"ZEB1 antibody validation"獲取,推薦關(guān)注《Nature Communications》《Cell Reports》等期刊近年研究。

       

背景信息

ZEB1 (zinc finger E-box-binding homeobox 1) is a transcription factor belonging to the Zinc finger E-box binding (ZEB) protein family, known for its critical role in epithelial-mesenchymal transition (EMT). EMT is a biological process involved in embryonic development, wound healing, and cancer metastasis, where epithelial cells lose adhesion and gain migratory properties. ZEB1 suppresses epithelial markers (e.g., E-cadherin) and promotes mesenchymal markers (e.g., vimentin), facilitating cell invasion and metastasis in cancers like breast, lung, and colorectal carcinomas.

ZEB1 antibodies are essential tools for detecting and quantifying ZEB1 protein expression in research. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. These antibodies are typically raised against specific epitopes of human ZEB1. with common host species including rabbit, mouse, and goat. Both monoclonal and polyclonal variants are available, each offering distinct advantages: monoclonal antibodies provide high specificity, while polyclonal antibodies may enhance sensitivity for detecting low-abundance targets.

Validation of ZEB1 antibodies is crucial, as cross-reactivity with homologous proteins (e.g., ZEB2) or non-specific binding can affect data reliability. Researchers often use knockout cell lines or siRNA-mediated ZEB1 knockdown to confirm antibody specificity. In cancer research, ZEB1 expression is studied to assess EMT progression, tumor aggressiveness, and therapeutic resistance. Commercial ZEB1 antibodies are often accompanied by validation data from peer-reviewed studies, ensuring reproducibility across experimental models.

       
關(guān)鍵字: ZEB1抗體;ZEB1;ZEB1 Antibody;

公司簡介

湖北瑞和寧生物科技有限公司研究創(chuàng)新能力, 擁有完整、科學(xué)、質(zhì)量管理體系。并不斷致力于技術(shù)創(chuàng)新,產(chǎn)品創(chuàng)新和管理創(chuàng)新,贏得了國內(nèi)外廣大用戶的信任。
成立日期 2024-06-24 (3年) 注冊資本 50萬(元)
員工人數(shù) 1-10人 年營業(yè)額 ¥ 100萬以內(nèi)
主營行業(yè) 中間體,化學(xué)試劑 經(jīng)營模式 貿(mào)易,工廠,試劑,定制,服務(wù)
  • 湖北瑞和寧生物科技有限公司
普通會員
  • 公司成立:3年
  • 注冊資本:50萬(元)
  • 企業(yè)類型:有限責(zé)任公司(自然人投資或控股)
  • 主營產(chǎn)品:中間體。試劑
  • 公司地址:江漢區(qū)
詢盤

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