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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Immunofluorescence analysis of A549 cells using IRAK3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

The IRAK3 (Interleukin-1 Receptor-Associated Kinase 3) antibody is a tool used to study the role of IRAK3. a key regulator in innate immune signaling. IRAK3. also known as IRAK-M, is a member of the IRAK family and functions as a negative feedback modulator of inflammatory responses. Unlike other IRAK kinases, IRAK3 lacks kinase activity and acts as a pseudokinase to inhibit signaling pathways downstream of Toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs). It suppresses NF-κB and MAPK activation, limiting excessive inflammation and maintaining immune homeostasis.

IRAK3 antibodies are essential for detecting IRAK3 expression in research models, including Western blotting, immunohistochemistry, and flow cytometry. Studies focus on its involvement in diseases such as sepsis, autoimmune disorders, and cancer, where dysregulated IRAK3 expression correlates with immune evasion or chronic inflammation. For example, IRAK3 upregulation in tumors may promote immunosuppression, while its deficiency can exacerbate inflammatory conditions.

These antibodies help elucidate IRAK3’s structure-function relationships, including its interaction with MyD88 and TRAF6 in TLR signaling. Researchers also explore IRAK3 as a therapeutic target, aiming to modulate its activity in inflammatory diseases or cancer immunotherapy. Its tissue-specific expression patterns and post-translational modifications further underscore the importance of reliable antibodies for precise experimental validation.