| 中文名稱:CBX4抗體 | 英文名稱:Mouse Monoclonal CBX4 Antibody |
| 品牌: 艾普蒂 | 產(chǎn)地: 武漢 |
| 保存條件: Stored at -20°C for 755 year. Avoid repeated freeze / thaw cycles. | 產(chǎn)品類別: 抗體 |
| 重組: 否 | 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA |
| 種屬反應(yīng)性: Human,Mouse,Rat | 宿主: Mouse |
| 偶聯(lián)物: 無 | 靶點: CBX4 |
| WB | 咨詢技術(shù) | Human,Mouse,Rat |
| IF | 咨詢技術(shù) | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技術(shù)咨詢 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PC2; NBP16 |
| Entrez GeneID | 8535 |
| clone | 6C5G3 |
| WB Predicted band size | 61.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CBX4 (AA: 397-514) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Flow cytometric analysis of Hela cells using CBX4 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using CBX4 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using CBX4 mouse mAb with DAB staining.
以下是關(guān)于CBX4抗體的3篇參考文獻及其摘要概括:
1. **文獻名稱**: "CBX4 Mediates HIF-1α-Dependent Tumor Metastasis in Hepatocellular Carcinoma"
**作者**: Liu et al.
**摘要**: 該研究揭示了CBX4通過調(diào)控HIF-1α的穩(wěn)定性促進肝癌轉(zhuǎn)移的機制,并開發(fā)了特異性CBX4抗體用于檢測其在腫瘤組織中的表達,證實其與患者預(yù)后不良相關(guān)。
2. **文獻名稱**: "Polycomb Protein CBX4 Sustains Embryonic Stem Cell Pluripotency through SUMO Modification"
**作者**: Zhang et al.
**摘要**: 本文利用CBX4抗體進行染色質(zhì)免疫沉淀實驗,發(fā)現(xiàn)CBX4通過SUMO化修飾維持胚胎干細胞多能性,并參與Polycomb復(fù)合體對關(guān)鍵基因的轉(zhuǎn)錄抑制。
3. **文獻名稱**: "CBX4 Coordinates the DNA Damage Response via Ubiquitin Signaling"
**作者**: Wang et al.
**摘要**: 研究通過CBX4抗體的蛋白質(zhì)互作分析,揭示了CBX4在DNA損傷修復(fù)中的作用,其通過調(diào)控泛素化信號通路促進修復(fù)因子募集至損傷位點,影響基因組穩(wěn)定性。
這些文獻均涉及CBX4抗體的應(yīng)用,涵蓋癌癥、干細胞及DNA修復(fù)等研究領(lǐng)域。
The CBX4 antibody is a crucial tool for studying the Chromobox homolog 4 (CBX4) protein, a member of the Polycomb group (PcG) family. CBX4. also known as hPc2. is a component of the Polycomb repressive complex 1 (PRC1), which plays a central role in epigenetic gene regulation by maintaining transcriptional repression of target genes, particularly through chromatin modification. CBX4 contains a conserved chromodomain that binds to trimethylated histone H3 lysine 27 (H3K27me3), a hallmark of PRC2-mediated silencing, linking PRC1 and PRC2 activities in establishing stable gene repression. Beyond its role in PRC1. CBX4 uniquely functions as a small ubiquitin-like modifier (SUMO) E3 ligase, regulating processes like DNA damage response, senescence, and stem cell pluripotency via SUMOylation of substrates such as HIPK2 or ΔNp63α.
CBX4 antibodies are widely used in research to investigate its expression, localization, and molecular interactions in developmental processes, cancer, and aging. They enable techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and immunohistochemistry to map CBX4’s tissue-specific distribution, nuclear dynamics, and association with chromatin. Studies using these antibodies have revealed CBX4’s dual roles in oncogenesis, where it acts as both a tumor suppressor (e.g., in squamous cell carcinoma) and a promoter of metastasis (e.g., in liver cancer), highlighting its context-dependent functions. Validated CBX4 antibodies are critical for exploring its therapeutic potential and mechanistic insights into epigenetic regulation, cellular differentiation, and disease progression.
| 成立日期 | 2024-06-24 (3年) | 注冊資本 | 50萬(元) |
| 員工人數(shù) | 1-10人 | 年營業(yè)額 | ¥ 100萬以內(nèi) |
| 主營行業(yè) | 中間體,化學試劑 | 經(jīng)營模式 | 貿(mào)易,工廠,試劑,定制,服務(wù) |
| 產(chǎn)品名稱 | 價格 | 公司名稱 | 報價日期 | |
|---|---|---|---|---|
| ¥1138 |
VIP4年
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2026-07-29 | |
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北京索萊寶科技有限公司
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2026-07-29 | |
| ¥1300 |
VIP1年
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2026-07-29 |