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Phospho-4E-BP1 (Ser65)抗體,Mouse Monoclonal Phospho-4E-BP1(Ser65) Antibody
  • Phospho-4E-BP1 (Ser65)抗體,Mouse Monoclonal Phospho-4E-BP1(Ser65) Antibody
  • Phospho-4E-BP1 (Ser65)抗體,Mouse Monoclonal Phospho-4E-BP1(Ser65) Antibody
  • Phospho-4E-BP1 (Ser65)抗體,Mouse Monoclonal Phospho-4E-BP1(Ser65) Antibody

Phospho-4E-BP1 (Ser65)抗體

價格 詢價
包裝 20μl 50μl 100μl
最小起訂量 1μl
發(fā)貨地 湖北
更新日期 2025-05-10
QQ交談 微信洽談

產(chǎn)品詳情

中文名稱:Phospho-4E-BP1 (Ser65)抗體英文名稱:Mouse Monoclonal Phospho-4E-BP1(Ser65) Antibody
品牌: 艾普蒂產(chǎn)地: 武漢
保存條件: Stored at -20°C for 936 year. Avoid repeated freeze / thaw cycles.產(chǎn)品類別: 抗體
重組: 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA
種屬反應(yīng)性: Human,Mouse,Rat 宿主: Mouse
偶聯(lián)物: 靶點: Phospho-4E-BP1 (Ser65)
2025-05-10 Phospho-4E-BP1 (Ser65)抗體 Mouse Monoclonal Phospho-4E-BP1(Ser65) Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武漢 Stored at -20°C for 936 year. Avoid repeated freeze / thaw cycles. 抗體

驗證與應(yīng)用

應(yīng)用及物種
WB咨詢技術(shù) Human,Mouse,Rat
IF咨詢技術(shù) Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/100 - 1/500 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

產(chǎn)品詳情

AliasesEIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
Entrez GeneID1978
clone1E2E7
WB Predicted band size12.6kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenSynthesized peptide of human Phospho-4E-BP1 (Ser65).
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Immunofluorescence analysis of Hela cells using Phospho-4E-BP1 (Ser65) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of Jurkat cells using Phospho-4E-BP1 (Ser65) mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.    


           

參考文獻

1. **"mTORC1 phosphorylates the translational repressor 4E-BP1 to regulate cell proliferation"**

- 作者:Gingras, A.C., et al.

- 摘要:研究通過質(zhì)譜和抗體驗證4E-BP1 Ser65磷酸化是mTORC1信號通路的關(guān)鍵事件,揭示其通過解除eIF4E抑制促進細胞周期進程和腫瘤生長的機制。

2. **"Targeting the mTOR-4E-BP1 axis in cancer therapy"**

- 作者:Hsieh, A.C., et al.

- 摘要:利用Phospho-4E-BP1 (Ser65)抗體評估多種癌細胞模型中的mTOR活性,發(fā)現(xiàn)抑制該磷酸化位點可增強化療敏感性,為聯(lián)合治療提供依據(jù)。

3. **"Regulation of 4E-BP1 phosphorylation by nutrient availability in mammalian cells"**

- 作者:Wang, X., et al.

- 摘要:通過免疫印跡和免疫熒光驗證氨基酸缺乏導致Ser65磷酸化水平下降,證明該抗體在代謝應(yīng)激研究中的特異性,關(guān)聯(lián)營養(yǎng)信號與蛋白質(zhì)合成調(diào)控。

4. **"Phosphorylation dynamics of 4E-BP1 in response to growth factors"**

- 作者:Thoreen, C.C., et al.

- 摘要:研究EGF和胰島素刺激下4E-BP1 Ser65磷酸化的時間依賴性變化,使用該抗體揭示其動態(tài)修飾在細胞生長和存活中的核心作用。

以上文獻涵蓋基礎(chǔ)機制、疾病模型和治療應(yīng)用,均通過Phospho-4E-BP1 (Ser65)抗體驗證目標蛋白的功能及調(diào)控網(wǎng)絡(luò)。

       

背景信息

The Phospho-4E-BP1 (Ser65) antibody detects the phosphorylated form of the eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) at serine 65. a key regulatory site in cellular translation control. 4E-BP1 inhibits cap-dependent translation by binding to the eukaryotic initiation factor 4E (eIF4E), preventing its interaction with eIF4G to form the eIF4F complex. Phosphorylation of 4E-BP1. particularly at Ser65. disrupts this interaction, releasing eIF4E to promote ribosome recruitment and protein synthesis. This phosphorylation event is primarily mediated by the mechanistic target of rapamycin complex 1 (mTORC1) in response to growth factors, nutrients, or cellular stress, linking it to pathways regulating cell growth, proliferation, and survival.

The Phospho-4E-BP1 (Ser65) antibody is widely used in research to assess mTORC1 activity and downstream signaling in conditions such as cancer, metabolic disorders, and aging. It helps evaluate therapeutic responses to mTOR inhibitors (e.g., rapamycin) or metabolic interventions. Specificity for the phosphorylated Ser65 epitope ensures accurate detection of the active, mTOR-regulated form of 4E-BP1. Applications include Western blotting, immunofluorescence, and immunohistochemistry, often paired with total 4E-BP1 antibodies for normalization. Proper sample preparation (e.g., phosphatase inhibitors) and timing are critical, as phosphorylation is dynamic and context-dependent. This antibody remains a vital tool for studying translational regulation and mTOR pathway dysregulation in disease.

       
關(guān)鍵字: Phospho-4E-BP1 (Ser6;Phospho-4E-BP1 (Ser6;Phospho-4E-BP1 (Ser6;

公司簡介

湖北瑞和寧生物科技有限公司研究創(chuàng)新能力, 擁有完整、科學、質(zhì)量管理體系。并不斷致力于技術(shù)創(chuàng)新,產(chǎn)品創(chuàng)新和管理創(chuàng)新,贏得了國內(nèi)外廣大用戶的信任。
成立日期 2024-06-24 (2年) 注冊資本 50萬(元)
員工人數(shù) 1-10人 年營業(yè)額 ¥ 100萬以內(nèi)
主營行業(yè) 中間體,化學試劑 經(jīng)營模式 貿(mào)易,工廠,試劑,定制,服務(wù)
  • 湖北瑞和寧生物科技有限公司
普通會員
  • 公司成立:2年
  • 注冊資本:50萬(元)
  • 企業(yè)類型:有限責任公司(自然人投資或控股)
  • 主營產(chǎn)品:中間體。試劑
  • 公司地址:江漢區(qū)
詢盤

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