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ATPIF1抗體,Mouse Monoclonal ATPIF1 Antibody
  • ATPIF1抗體,Mouse Monoclonal ATPIF1 Antibody
  • ATPIF1抗體,Mouse Monoclonal ATPIF1 Antibody
  • ATPIF1抗體,Mouse Monoclonal ATPIF1 Antibody

ATPIF1抗體

價(jià)格 詢價(jià)
包裝 20μl 50μl 100μl
最小起訂量 1μl
發(fā)貨地 湖北
更新日期 2025-05-10
QQ交談 微信洽談

產(chǎn)品詳情

中文名稱:ATPIF1抗體英文名稱:Mouse Monoclonal ATPIF1 Antibody
品牌: 艾普蒂產(chǎn)地: 武漢
保存條件: Stored at -20°C for 984 year. Avoid repeated freeze / thaw cycles.產(chǎn)品類別: 抗體
重組: 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA
種屬反應(yīng)性: Human,Mouse,Rat 宿主: Mouse
偶聯(lián)物: 靶點(diǎn): ATPIF1
2025-05-10 ATPIF1抗體 Mouse Monoclonal ATPIF1 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武漢 Stored at -20°C for 984 year. Avoid repeated freeze / thaw cycles. 抗體

驗(yàn)證與應(yīng)用

應(yīng)用及物種
WB咨詢技術(shù) Human,Mouse,Rat
IF咨詢技術(shù) Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/100 - 1/500 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

產(chǎn)品詳情

AliasesIP; ATPI; ATPIP
Entrez GeneID93974
clone1F3B8
WB Predicted band size12.3kDa
Host/IsotypeMouse IgG2b
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human ATPIF1 (AA: 1-106) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Immunofluorescence analysis of Hela cells using ATPIF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of Hela cells using ATPIF1 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using ATPIF1 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using ATPIF1 mouse mAb with DAB staining.    


           

參考文獻(xiàn)

以下是關(guān)于ATPIF1抗體的3篇參考文獻(xiàn)示例(注:文獻(xiàn)為虛擬示例,僅供參考格式):

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1. **文獻(xiàn)名稱**: *"ATPIF1 regulates mitochondrial apoptosis in cancer cells via interaction with cyclophilin D"*

**作者**: Chen, L. et al.

**摘要**: 研究通過ATPIF1抗體進(jìn)行免疫共沉淀和Western blot分析,揭示了ATPIF1通過與Cyclophilin D相互作用調(diào)控線粒體膜通透性,從而影響癌細(xì)胞凋亡的分子機(jī)制。

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2. **文獻(xiàn)名稱**: *"Development of a specific monoclonal antibody for ATPIF1 and its application in metabolic disorder studies"*

**作者**: Sato, K. et al.

**摘要**: 本文報(bào)道了一種高特異性ATPIF1單克隆抗體的開發(fā),并通過免疫組化和流式細(xì)胞術(shù)驗(yàn)證其在肝組織中的表達(dá),探討ATPIF1在代謝綜合征中的潛在作用。

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3. **文獻(xiàn)名稱**: *"ATPIF1 knockdown impairs mitochondrial function and triggers ROS production: Evidence from ATPIF1 antibody-based assays"*

**作者**: Gomez, M. et al.

**摘要**: 利用ATPIF1抗體進(jìn)行蛋白質(zhì)表達(dá)敲低驗(yàn)證及亞細(xì)胞定位分析,發(fā)現(xiàn)ATPIF1缺失導(dǎo)致線粒體活性氧(ROS)累積,提示其在氧化應(yīng)激中的關(guān)鍵調(diào)控功能。

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**提示**:實(shí)際文獻(xiàn)需通過PubMed、Google Scholar等平臺檢索關(guān)鍵詞“ATPIF1 antibody”或“ATPIF1 + 應(yīng)用技術(shù)(如Western blot)”,并篩選涉及抗體開發(fā)或?qū)嶒?yàn)應(yīng)用的原創(chuàng)研究。建議結(jié)合研究主題補(bǔ)充具體文獻(xiàn)。

       

背景信息

ATPIF1 (ATPase inhibitory factor 1), also known as ATPIF1 or IF1. is a mitochondrial protein that regulates the activity of ATP synthase, the enzyme responsible for generating ATP during oxidative phosphorylation. It functions as an endogenous inhibitor of ATP hydrolysis, preventing the reverse operation of ATP synthase under conditions of low pH or membrane potential, thereby conserving cellular ATP levels during energy stress. Dysregulation of ATPIF1 has been implicated in metabolic disorders, cancer, and neurodegenerative diseases, as it influences mitochondrial bioenergetics and cellular survival pathways.

Antibodies targeting ATPIF1 are essential tools for studying its expression, localization, and functional roles in various biological contexts. These antibodies enable researchers to detect ATPIF1 protein levels in tissues or cell lines using techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). They are particularly valuable in investigating mitochondrial dysfunction, metabolic adaptation in tumors, and cellular responses to hypoxia or nutrient deprivation. Validation of ATPIF1 antibodies typically includes specificity checks using knockout cell models or siRNA-mediated knockdown. Commercial ATPIF1 antibodies are often produced in hosts like rabbits or mice, with options for monoclonal or polyclonal formats. Their applications span basic research in mitochondrial biology, as well as translational studies exploring ATPIF1 as a potential therapeutic target in diseases linked to energy metabolism imbalances.

       
關(guān)鍵字: ATPIF1抗體;ATPIF1;ATPIF1 Antibody;

公司簡介

湖北瑞和寧生物科技有限公司研究創(chuàng)新能力, 擁有完整、科學(xué)、質(zhì)量管理體系。并不斷致力于技術(shù)創(chuàng)新,產(chǎn)品創(chuàng)新和管理創(chuàng)新,贏得了國內(nèi)外廣大用戶的信任。
成立日期 2024-06-24 (2年) 注冊資本 50萬(元)
員工人數(shù) 1-10人 年?duì)I業(yè)額 ¥ 100萬以內(nèi)
主營行業(yè) 中間體,化學(xué)試劑 經(jīng)營模式 貿(mào)易,工廠,試劑,定制,服務(wù)
  • 湖北瑞和寧生物科技有限公司
普通會員
  • 公司成立:2年
  • 注冊資本:50萬(元)
  • 企業(yè)類型:有限責(zé)任公司(自然人投資或控股)
  • 主營產(chǎn)品:中間體。試劑
  • 公司地址:江漢區(qū)
詢盤

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ATPIF1抗體相關(guān)廠家報(bào)價(jià)

產(chǎn)品名稱 價(jià)格   公司名稱 報(bào)價(jià)日期
詢價(jià)
上海嘉定區(qū)澄瀏公路52號
2025-11-06
¥1600.00
VIP10年
北京索萊寶科技有限公司
2026-06-02
¥1300
VIP1年
上海博湖生物科技有限公司
2026-06-03
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