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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Mouse kidney tissue, Primary antibody: P01308(EEF1AKMT4 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P01308(EEF1AKMT4 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The EEF1AKMT4 antibody is a research tool designed to detect and study the eukaryotic elongation factor 1A lysine methyltransferase 4 (EEF1AKMT4), also known as METTL21D. This enzyme catalyzes the methylation of eukaryotic translation elongation factor 1A (eEF1A), a critical component of the protein synthesis machinery. Specifically, EEF1AKMT4 methylates lysine residues (e.g., K318 in humans) on eEF1A, a post-translational modification implicated in regulating translation fidelity, stress response, and protein-protein interactions during cellular processes.

The antibody is typically developed in immunized hosts (e.g., rabbits) and validated for specificity through techniques like Western blotting, immunofluorescence, or immunoprecipitation. It enables researchers to investigate EEF1AKMT4 expression patterns, subcellular localization, and its role in diseases such as cancers or neurodegenerative disorders, where dysregulated translation and protein homeostasis are observed. Studies using this antibody contribute to understanding how methylation modulates eEF1A’s functions in cell proliferation, apoptosis, and adaptation to environmental stress. Its application aids in elucidating the broader significance of post-translational modifications in translational control and disease mechanisms.