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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: MCF7 cell lysate, Primary antibody: P01733(PCGF6 Antibody) at dilution 1/1150, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 minute    

  
  

The PCGF6 antibody is a research tool designed to detect Polycomb Group RING Finger 6 (PCGF6), a component of the Polycomb Repressive Complex 1 (PRC1) family. PCGF6. also known as MBLR or MEL18. plays a role in epigenetic regulation by facilitating transcriptional repression through histone H2A ubiquitination. It is part of non-canonical PRC1 complexes that interact with distinct sets of proteins, such as L3MBTL2 or E2F6. to regulate gene silencing in a context-dependent manner. PCGF6 is implicated in embryonic development, stem cell pluripotency, and cellular differentiation, with studies linking its dysfunction to oncogenesis and developmental disorders.

The antibody is widely used in techniques like Western blotting, immunoprecipitation, and chromatin immunoprecipitation (ChIP) to study PCGF6's expression, localization, and interactions. Researchers employ it to explore its role in repressing lineage-specific genes in embryonic stem cells and its regulatory effects on cell cycle progression. Validation often includes testing in PCGF6-knockout cell lines to confirm specificity. Challenges include distinguishing PCGF6 from other Polycomb family members due to structural similarities. Recent studies also highlight its potential involvement in X-chromosome inactivation and metabolic reprogramming in cancer, making this antibody critical for advancing epigenetic and disease-related research.