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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Human bladder carcinoma tissue, Primary antibody: P01799(SRP54 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using P01799(SRP54 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

The SRP54 antibody is a research tool targeting the Signal Recognition Particle 54 kDa (SRP54) protein, a critical component of the signal recognition particle (SRP) complex. SRP is a ribonucleoprotein complex conserved across eukaryotes and archaea, responsible for co-translational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). SRP54. encoded by the *SRP54* gene, contains a GTPase domain and a methionine-rich domain that binds the SRP RNA and signal sequences of nascent polypeptides. It plays a pivotal role in recognizing signal peptides, pausing translation, and directing ribosome-nascent chain complexes to the ER membrane via interaction with the SRP receptor.

Antibodies against SRP54 are widely used in molecular biology to study SRP assembly, protein translocation mechanisms, and ER-related diseases. Dysregulation of SRP54 has been linked to congenital disorders like severe neutropenia, pancreatic insufficiency, and skeletal dysplasia due to mutations affecting SRP function. Autoantibodies targeting SRP54 are also observed in certain autoimmune conditions, including immune-mediated necrotizing myopathy. Researchers employ SRP54 antibodies in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate its expression, localization, and interactions. Commercial antibodies are typically validated for specificity using knockout cell lines or siRNA knockdowns to ensure accurate detection in disease models or basic research on protein trafficking pathways.