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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: A549 cells, Primary antibody: P05202(CIZ1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

The CIZ1 (Cip1-interacting zinc finger protein 1) antibody is a tool used to detect and study the CIZ1 protein, a nuclear matrix-associated factor involved in DNA replication, cell cycle regulation, and transcriptional control. CIZ1 interacts with p21Cip1/WAF1. a cyclin-dependent kinase inhibitor, to modulate cell proliferation and differentiation. It is implicated in various cancers, including breast, liver, and lung cancers, where its dysregulated expression correlates with tumor progression, metastasis, and poor prognosis. CIZ1 exists in multiple isoforms generated by alternative splicing, with tissue-specific functions, and its activity is regulated by post-translational modifications like phosphorylation.

CIZ1 antibodies, including monoclonal and polyclonal variants, are widely employed in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression, localization, and isoform-specific roles. Their specificity is critical due to CIZ1’s structural complexity and homology with other zinc finger proteins. Validation via knockout cells or siRNA-mediated depletion is often required. These antibodies aid in exploring CIZ1’s dual roles in cancer—acting as an oncogene in certain contexts while suppressing metastasis in others—and its potential as a diagnostic biomarker or therapeutic target. Challenges in antibody development include distinguishing functional isoforms and minimizing cross-reactivity.