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     The image is immunohistochemistry of paraffin-embedded Human brain tissue using P05299(SLC25A12 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using P05299(SLC25A12 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

The SLC25A12 gene encodes the mitochondrial aspartate/glutamate carrier protein Aralar1 (AGC1), a member of the solute carrier family 25 (SLC25) responsible for transporting metabolites across mitochondrial membranes. This calcium-binding carrier plays a critical role in the malate-aspartate shuttle, facilitating the exchange of aspartate from the mitochondrial matrix with glutamate and protons from the cytosol. It is essential for maintaining cellular energy metabolism, urea cycle function, and neurotransmitter synthesis, particularly in the central nervous system.

Antibodies targeting SLC25A12 are widely used to study its expression, localization, and function in various tissues, especially in neurological and metabolic research. They enable detection of the protein via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Given SLC25A12's association with neurodevelopmental disorders, including autism spectrum disorder (ASD) and epileptic encephalopathy, these antibodies are valuable tools for investigating disease mechanisms.

Most commercial SLC25A12 antibodies are raised against specific epitopes, such as the N-terminal or C-terminal regions, and validated for reactivity in human, mouse, or rat samples. Researchers frequently employ them to examine protein expression changes in disease models or to confirm CRISPR/Cas9-mediated gene editing. Proper validation using knockout controls is critical due to potential cross-reactivity with related carriers like SLC25A13 (Aralar2). Studies often focus on its role in mitochondrial dysfunction and neuronal development.