| 中文名稱:ING2抗體 | 英文名稱:Rabbit Polyclonal ING2 Antibody |
| 品牌: 艾普蒂 | 產(chǎn)地: 武漢 |
| 保存條件: Stored at -20°C for 3513 year. Avoid repeated freeze / thaw cycles. | 產(chǎn)品類別: 抗體 |
| 重組: 否 | 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA |
| 種屬反應(yīng)性: Human,Mouse,Rat | 宿主: Rabbit |
| 偶聯(lián)物: 無(wú) | 靶點(diǎn): ING2 |
| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨詢技術(shù) | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技術(shù)咨詢 | Human,Mouse,Rat |
| FCM | 咨詢技術(shù) | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ING1L; p33ING2 |
| WB Predicted band size | 33 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ING2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Lovo cells, human liver cancer tissue, mouse kidney tissue, Primary antibody: P07382(ING2 Antibody) at dilution 1/425, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds
The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P07382(ING2 Antibody) at dilution 1/40. (Original magnification: ×200)
The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P07382(ING2 Antibody) at dilution 1/40. (Original magnification: ×200)
以下是3篇與ING2抗體相關(guān)的參考文獻(xiàn),按研究主題和內(nèi)容簡(jiǎn)要概括:
1. **"ING2 regulates chromatin structure and gene expression by modulating histone H3K4 methylation"**
*作者:Garkavtsev et al. (2004)*
**摘要**:研究揭示了ING2通過(guò)結(jié)合組蛋白H3K4me3并招募組蛋白去乙酰化酶(HDAC)復(fù)合體,調(diào)控染色質(zhì)結(jié)構(gòu)和基因表達(dá)的功能。實(shí)驗(yàn)使用ING2抗體進(jìn)行免疫沉淀和染色質(zhì)免疫共沉淀(ChIP),證實(shí)其在抑制腫瘤生長(zhǎng)中的關(guān)鍵作用。
2. **"The tumor suppressor ING2 mediates epigenetic regulation of cellular senescence"**
*作者:Thalappilly et al. (2010)*
**摘要**:通過(guò)免疫組織化學(xué)(IHC)和Western blot分析,研究證明ING2抗體檢測(cè)到的蛋白表達(dá)水平與細(xì)胞衰老和DNA損傷修復(fù)相關(guān)。ING2缺失導(dǎo)致表觀遺傳修飾異常,促進(jìn)腫瘤發(fā)生。
3. **"ING2 interacts with p300/CBP to enhance p53 acetylation and transcriptional activity"**
*作者:Kuo et al. (2008)*
**摘要**:文章利用ING2抗體進(jìn)行共聚焦顯微成像和免疫共沉淀實(shí)驗(yàn),發(fā)現(xiàn)ING2通過(guò)結(jié)合p300/CBP復(fù)合體增強(qiáng)p53的乙?;?,進(jìn)而激活促凋亡基因,抑制腫瘤進(jìn)展。
4. **"Loss of ING2 expression predicts poor prognosis in hepatocellular carcinoma"**
*作者:Unoki et al. (2011)*
**摘要**:臨床樣本分析中,ING2抗體的免疫組化結(jié)果顯示,ING2低表達(dá)與肝癌患者生存率下降顯著相關(guān),提示其作為預(yù)后生物標(biāo)志物的潛力。
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**備注**:以上文獻(xiàn)標(biāo)題和作者為模擬示例,實(shí)際引用需以真實(shí)出版物為準(zhǔn)。建議通過(guò)PubMed或Google Scholar以關(guān)鍵詞“ING2 antibody”、“ING2 function”或“ING2 cancer”檢索最新研究。
The ING2 (Inhibitor of Growth 2) antibody is a research tool used to detect and study the ING2 protein, a member of the ING family of tumor suppressors. ING2 is a nuclear protein involved in chromatin remodeling, transcriptional regulation, and cellular stress responses. It interacts with histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes, modulating histone acetylation status to influence gene expression. ING2 plays roles in cell cycle arrest, apoptosis, DNA repair, and senescence, often acting as a tumor suppressor by regulating p53-dependent pathways. Dysregulation of ING2 has been linked to cancers, neurodegenerative diseases, and aging.
The ING2 antibody is widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression, localization, and interactions in cell lines or tissues. Researchers utilize it to explore ING2's involvement in epigenetic regulation, cancer progression, and therapeutic responses. Specificity and validation (e.g., knockout controls) are critical due to potential cross-reactivity with other ING family members. Commercial antibodies often target epitopes within the N-terminal region or plant homeodomain (PHD) zinc finger, which are essential for ING2's chromatin-binding activity. Studies employing this antibody have revealed ING2's downregulation in certain malignancies and its role in oxidative stress responses, making it a focal point in both basic and translational cancer research.
| 成立日期 | 2024-06-24 (3年) | 注冊(cè)資本 | 50萬(wàn)(元) |
| 員工人數(shù) | 1-10人 | 年?duì)I業(yè)額 | ¥ 100萬(wàn)以內(nèi) |
| 主營(yíng)行業(yè) | 中間體,化學(xué)試劑 | 經(jīng)營(yíng)模式 | 貿(mào)易,工廠,試劑,定制,服務(wù) |
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