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     The image is immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using P08354(GNAI3 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

The GNAI3 antibody is a tool used to detect and study the G protein subunit alpha i3 (GNAI3), a member of the inhibitory G protein (Gi/o) family involved in intracellular signal transduction. GNAI3 functions as a molecular switch, cycling between active GTP-bound and inactive GDP-bound states to regulate downstream effectors such as adenylate cyclase, ion channels, and mitogen-activated protein kinase (MAPK) pathways. It plays critical roles in diverse physiological processes, including cell proliferation, differentiation, and immune response regulation. Dysregulation of GNAI3 has been implicated in pathological conditions such as cancer, inflammatory diseases, and neurological disorders.

GNAI3 antibodies are widely utilized in research applications like Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression, localization, and post-translational modifications. These antibodies are essential for investigating GNAI3's interaction networks, its role in GPCR-mediated signaling, and its potential as a therapeutic target. Specificity and validation (e.g., knockout controls) are crucial, as cross-reactivity with homologous Gα subunits (e.g., GNAI1. GNAI2) can occur. Commercially available antibodies are typically raised in hosts like rabbits or mice, with formats ranging from monoclonal to polyclonal. Recent studies highlight GNAI3's involvement in tumor metastasis and immune cell modulation, underscoring the antibody's relevance in translational and mechanistic research.