基于液滴的微流體技術是一種新興的強大工具，適用于dPCR或單細胞分析等生物應用。作為微反應器的液滴，可以在微流控芯片中產生，具有非常高的吞吐量，可在較短的時間內進行大量的平行實驗。

氟化油通常用作這類應用的連續(xù)相，因為其能夠溶解和輸送氧氣，并且不溶解大多數(shù)化學和生化化學物。

FluoSurf 是一種生物相容性氟化表面活性劑，專為生產單分散油包水液滴而設計和優(yōu)化。FluoSurf 可確保液滴的長期穩(wěn)定性（即使在極端條件下也是如此）。

優(yōu)勢

● 穩(wěn)定性

   即使在dPCR或長期細胞培養(yǎng)實驗等具有挑戰(zhàn)性的條件下，也能實現(xiàn)單分散液滴群體和低液滴融合。

● 生物相容性

   經(jīng)細胞檢測驗證的生物相容性，適用于生物實體。

● 批量生產

   我們的大批量生產能力使我們能夠滿足您的所有需求

● 較高純度

   在制造加工過程中去除雜質

● 泄露控制：液滴內的分散相物質較少量的滲透到液滴外部

● 批次間重現(xiàn)性

   每個批次都遵循嚴格的質量控制分析，通過驗證結構和微流體性能來確保一致性。

FluoSurf 在高溫處理（例如PCR的加熱循環(huán)）后仍保證液滴穩(wěn)定性和群體單分散性。

應用領域

● 數(shù)字液滴PCR

   基于對液滴內大量分隔PCR反應的統(tǒng)計分析的超靈敏核酸定量方法

● 單細胞分析

   在受控環(huán)境下處理和分析單分散液滴群體中的分離細胞

● 篩選

   在微滴內以高通量對細胞、基因或生物大分子進行受控操作、分析和分選。

● 合成生物學

   使用微液滴作為細胞大小的隔間來模擬代謝反應。

● 控制化學和生物反應

   微流體工具專門設計用于增強對封裝在液滴內的反應的控制。

規(guī)格參數(shù)

案例1: 液滴產生和熱循環(huán)對尺寸分布的影響

實驗條件：氟油流速為5μL/min，水相流速為1.66μL/min。

4w/w%表面活性劑HFE7500

熱循環(huán)條件：30個循環(huán)

95℃駐留30s

55℃駐留1min

72℃駐留5min

40℃駐留1min

熱循環(huán)前

熱循環(huán)后

FluoSurf在熱循環(huán)期間允許液滴具有良好的穩(wěn)定性

使用FluoSurf表面活性劑，熱循環(huán)對液滴尺寸分布的影響十分有限。

案例2：保留性能

熒光素100μM的全/空液滴平均強度的演變

FluoSurf限制液滴之間的交換（4天后少于10%的熒光素交換）

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