EGFR(G719S) [100 μL] Catalog Number:  CR-M26083 Gene Symbol:  EGFR Description:   Anti EGFR(G719S) Mouse Monoclonal Antibody Background:   EGFR is a receptor tyrosine kinase for ligands of the EGF family. EGFR activates several signaling cascades to convert extracellular cues into appropriate cellular responses. Defects in EGFR are associated with cancers such as lung cancer. Immunogen:   A synthetic peptide from the internal region of human EGFR which includes the mutation of G719S. Applications:   ELISA, WB, IHC, IF Recommended Dilutions:    ELISA: 1:1000-1:5000  WB: 1:1000-1:2000  IHC: 1:50-1:1000  IF: 1:50-1:100 Concentration:   1 mg/ml Host:   mouse Format:   Liquid Clonality:   Monoclonal Isotype:   IgG2b Purity:   Purified from ascites Storage buffer: Preservative:  no Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  Recognizes EGFR(G719S), but not wild type EGFR protein of vertebrates. Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles. Western blot:

Western blot analysis of recombinant EGFR(G719S) and wild-type EGFR proteins. Purified his-tagged EGFR(G719S) protein (aa 702-987) (Cat. #CR-P10214) and wild-type EGFR protein (aa 702-987) (Cat. #CR-P10210) were blotted with anti EGFR(G719S) mouse antibody (Cat. #CR-M26083).

Immunofluorescence:

Immunofluorescence of cells expressing EGFR proteins with anti EGFR(G719S) antibody. HEK293T cells were transfected with pCDNA3-GFP EGFR(WT) plasmid (left column) or pCDNA3-GFP EGFR(G719S) plasmid (right column), then fixed and stained with anti EGFR(G719S) monoclonal antibody (Cat. #CR-M26083).