描述(Description)
KGF/FGF-7 proteins coordinate embryonic development and regulate basic processes such as cell proliferation and differentiation. Its critical role extends to normal branching morphogenesis. KGF/FGF-7 Protein, Human (His) is the recombinant human-derived KGF/FGF-7 protein, expressed by E. coli , with N-His labeled tag.
生物活性(Bioactivity)
Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells.The ED50 for this effect is ≤110.3 ng/mL, corresponding to a specific activity is ≥9.07×103 U/mg.
氨基酸序列(Target Protein Sequence)
CNDMTPEQMATNVNCSSPERHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNNYNIMEIRTVAVGIVAIKGVESEFYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHNGGEMFVALNQKGIPVRGKKTKKEQKTAHFLPMAIT
制劑(Trait)
Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 1mM EDTA, 5% Trehalose, 0.02% Tween 80, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCL, pH 7.4 or PBS or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0, 5% trehalose, 5% mannitol and 0.01% Tween80 or 20 mM PB, 500 mM NaCl, pH 8.0, 8% trehalose.
復溶(Reconstitution)
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
保存條件&效期(Storage & Stability)
Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
運輸方式(Shipping)
Shipping with dry ice.

生物活性(Bioactivity)
Measured in a cell proliferation assay using Mv.1.Lu Mink lung epithelial cells. The ED50 for this effect is ≤10 ng/mL.

電泳(SDS-PAGE)
Greater than 95% as determined by reducing SDS-PAGE.
特別提示:本公司的所有產品僅用于工業(yè)應用或者科學研究等非醫(yī)療目的,不可用于人類或動物的臨床診斷或者治療,非藥用,非食用。
關鍵字: GMP級;高純度;批間一致;生物活性;科研專供;
在生物科技領域,豐度蛋白專注重組蛋白供應,以“豐度的風帆為蛋白提供動力,豐度為蛋白護航”為使命,賦能生命科學發(fā)展。
蘇州豐度依托先進技術平臺,打造成熟生產體系,嚴控基因工程設計、細胞培養(yǎng)、蛋白純化等環(huán)節(jié),優(yōu)化工藝與管理,確保產品高純度、高活性且批間一致。產品覆蓋基礎研究、生物醫(yī)藥研發(fā)等多領域,為科研及生產提供多樣、適配產品,助力突破技術難題 。