描述（Description）
Fibronectin binds collagen, fibrin, heparin, DNA, and actin. It is involved in cell adhesion, motility, opsonization, wound healing and maintenance of cell shape. GMP Fibronectin Protein, Human is a recombinant protein dimer complex containing human-derived Fibronectin protein, expressed by E. coli , with tag free. GMP Fibronectin Protein, Human, has molecular weight of ~60.0 kDa.
生物活性（Bioactivity）
1.Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is ≤ 385.7 ng/mL.
2.Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. The ED50 for this effect is ≤182.9 ng/mL, corresponding to a specific activity is ≥5.467×103 units/mg.
氨基酸序列（Target Protein Sequence）
PTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPS&AIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPST
制劑（Trait）
Lyophilized from a 0.2 μm filtered solution of 12.5 mM Sodium Citrate, 1.25% Sucrose, pH 5.5 or PBS, pH 6.8, 5% trehalose, 5% mannitol and 0.01% Tween 80.
復(fù)溶（Reconstitution）
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
保存條件&效期（Storage & Stability）
Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
運(yùn)輸方式(Shipping)
Room temperature in continental US; may vary elsewhere.
生物活性（Bioactivity）
Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. The ED50 for this effect is 124.5 ng/mL, corresponding to a specific activity is 8.03×103 units/mg.
電泳（SDS-PAGE）
Greater than 95% as determined by reducing SDS-PAGE.
特別提示：本公司的所有產(chǎn)品僅用于工業(yè)應(yīng)用或者科學(xué)研究等非醫(yī)療目的,不可用于人類或動物的臨床診斷或者治療,非藥用,非食用。
關(guān)鍵字： GMP級;高純度;批間一致;生物活性;科研專供;
在生物科技領(lǐng)域，豐度蛋白專注重組蛋白供應(yīng)，以“豐度的風(fēng)帆為蛋白提供動力，豐度為蛋白護(hù)航”為使命，賦能生命科學(xué)發(fā)展。
蘇州豐度依托先進(jìn)技術(shù)平臺，打造成熟生產(chǎn)體系，嚴(yán)控基因工程設(shè)計(jì)、細(xì)胞培養(yǎng)、蛋白純化等環(huán)節(jié)，優(yōu)化工藝與管理，確保產(chǎn)品高純度、高活性且批間一致。產(chǎn)品覆蓋基礎(chǔ)研究、生物醫(yī)藥研發(fā)等多領(lǐng)域，為科研及生產(chǎn)提供多樣、適配產(chǎn)品，助力突破技術(shù)難題 。