Human EGF R Protein, His Tag (MALS verified)

分子別名（Synonym）

EGFR,ERBB,ERBB1,HER1,PIG61,mENA

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human EGF R, His Tag (EGR-H5222) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).

Predicted N-terminus: Leu 25

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 70.5 kDa. The protein migrates as 95-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介紹

表皮生長(zhǎng)因子受體（EGFR，又稱(chēng)ErbB-1或人源HER1）是細(xì)胞表面受體，特異性結(jié)合表皮生長(zhǎng)因子家族（EGF家族）的胞外蛋白配體。該受體作為ErbB受體家族成員，屬于由四種高度同源的受體酪氨酸激酶組成的亞家族：EGFR（ErbB-1）、HER2/c-neu（ErbB-2）、HER3（ErbB-3）及HER4（ErbB-4）。影響EGFR表達(dá)或活性的基因突變可能誘發(fā)癌癥。