Human FOLR1 Protein, His Tag (MALS verified)

分子別名（Synonym）

FOLR-1, FBP, FOLR, FRα

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human FOLR1, His Tag (FO1-H52H1) is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Met 233 (Accession # P15328-1).

Predicted N-terminus: Arg 25

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 26.5 kDa. The protein migrates as 32-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method / rFC method.

純度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景介紹

葉酸受體1（FOLR1）——亦稱葉酸受體α、葉酸結(jié)合蛋白（FBP）、FOLR——是葉酸受體家族成員。該基因家族對葉酸及其多種還原衍生物具有高親和力，負(fù)責(zé)介導(dǎo)5-甲基四氫葉酸向胞內(nèi)的轉(zhuǎn)運。成熟FOLR1為N-糖基化蛋白，通過GPI錨定于細(xì)胞表面，主要在上皮細(xì)胞表達(dá)且在多種癌變組織中顯著高表達(dá)。該受體經(jīng)內(nèi)化進入內(nèi)體系統(tǒng)后與配體解離，最終循環(huán)至細(xì)胞膜表面。其可溶形式可通過酶切脫落進入血清及乳汁。FOLR1基因缺陷將導(dǎo)致腦葉酸轉(zhuǎn)運缺陷性神經(jīng)退行性病變（NCFTD），這是一種因嬰幼兒期腦組織特異性葉酸缺乏引發(fā)的常染色體隱性遺傳病。