Cynomolgus CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)

分子別名（Synonym）

CD3E & CD3D,CD3 delta & CD3 epsilon

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (CDD-C52W4) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # Q95LI5 (CD3E) & Q95LI8 (CD3D)).

Predicted N-terminus: Gln 22 (CD3E) & Phe 22 (CD3D)

蛋白結(jié)構(gòu)（Molecular Characterization）

Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 15.5 kDa (CD3E) and 14.5 kDa (CD3D). Subunit CD3E is fused with his tag at the C-terminus and subunit CD3D is fused with flag tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) and Phe 22 (CD3D). The reducing (R) heterodimer protein migrates as 15kDa, 17kDa, 27-32kDa when calibrated against Star Ribbon Pre-stained Protein Marker due to glyosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

純度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介紹

T細(xì)胞表面糖蛋白CD3δ鏈和CD3ε鏈（分別簡稱CD3D與CD3E或CD3D&CD3E）屬于單次跨膜I型膜蛋白。CD3δ鏈與CD3ε鏈、CD3γ鏈、CD3ζ鏈以及T細(xì)胞受體α/β和γ/δ異源二聚體共同構(gòu)成T細(xì)胞受體-CD3復(fù)合物。該復(fù)合物在將抗原識別信號偶聯(lián)至多條細(xì)胞內(nèi)信號轉(zhuǎn)導(dǎo)通路的過程中發(fā)揮關(guān)鍵作用。