Human EGF Protein, His Tag

分子別名（Synonym）

EGF, URG, HOMG4

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human EGF Protein, His Tag (EGF-H52H3) is expressed from human 293 cells (HEK293). It contains AA Asn 971 - Arg 1023 (Accession # P01133-1).

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 8.3 kDa. The protein migrates as 10-11 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method / rFC method.

無(wú)菌（Sterility）

Negative

支原體（Mycoplasma）

Negative

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介紹

人表皮生長(zhǎng)因子（EGF）亦稱(chēng)HOMG4或URG，是一種通過(guò)與其受體EGFR結(jié)合，在細(xì)胞生長(zhǎng)、增殖和分化調(diào)節(jié)中發(fā)揮重要作用的生長(zhǎng)因子。該因子存在于人類(lèi)血小板、巨噬細(xì)胞、尿液、唾液、乳汁及血漿中。EGF是EGF蛋白家族的創(chuàng)始成員，該家族各成員具有高度相似的結(jié)構(gòu)與功能特征——所有成員均包含一個(gè)或多個(gè)保守氨基酸序列重復(fù)單元。

唾液EGF的生物學(xué)效應(yīng)包括：促進(jìn)口腔及胃食管潰瘍愈合、抑制胃酸分泌、刺激維生素D合成，以及對(duì)黏膜的保護(hù)作用（抵御胃酸、膽汁酸、胰蛋白酶、胰凝乳蛋白酶等管腔內(nèi)損傷因子，以及物理、化學(xué)和細(xì)菌性侵害）。由于EGF會(huì)增加癌癥風(fēng)險(xiǎn)，抑制其活性可降低患癌風(fēng)險(xiǎn)。