PE-Labeled Recombinant Protein L, His Tag (Site-specific conjugation)

分子別名（Synonym）

RPL,Protein L

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

PE-Labeled Recombinant Protein L, His Tag (RPL-PP2H2) is produced via site-specific conjugation of PE to Recombinant Protein L, His Tag under optimal conditions with a proprietary technology. Recombinant Protein L, His Tag is expressed from E. coli cells. It contains AA Lys 106 - Gly 470 (Accession # D6S9W1-1).

Predicted N-terminus: His

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 43.7 kDa.

偶聯(lián)（Conjugate）

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

應(yīng)用說明（Application）

Please note that this product is NOT compatible to streptavidin detection system.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介紹

蛋白質(zhì)L源自大消化鏈球菌（Peptostreptococcus magnus）表面，因其通過與免疫球蛋白（IgG、IgM、IgA、IgE及IgD）的L鏈結(jié)合的特性而得名。盡管具有廣泛的免疫球蛋白類別結(jié)合能力，該蛋白并非通用型免疫球蛋白結(jié)合蛋白——其結(jié)合特異性局限于含有κ輕鏈（即VL結(jié)構(gòu)域的κ鏈）的免疫球蛋白。人類和小鼠的免疫球蛋白以κ輕鏈為主，其余免疫球蛋白則含有λ輕鏈。該重組蛋白包含天然蛋白的四個免疫球蛋白（Ig）結(jié)合結(jié)構(gòu)域（B結(jié)構(gòu)域）。除完整抗體外，蛋白質(zhì)L還可廣泛結(jié)合Fab片段、單鏈可變片段（scFv）及結(jié)構(gòu)域抗體（Dabs）等多種抗體片段。