Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein, His Tag&Tag Free
分子別名（Synonym）
Integrin alpha 4 beta 1, ITGA4&ITGB1
表達區(qū)間及表達系統(tǒng)（Source）
Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52W1) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1) (Accession # P13612-1 (ITGA4) & P05556-1 (ITGB1)).
Predicted N-terminus: Tyr 34 & Ser 592 (ITGA4) & Gln 21 (ITGB1)
蛋白結構（Molecular Characterization）
Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA4 and ITGB1, has a calculated MW of 111.5 kDa (ITGA4) and 83.7 kDa (ITGB1). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The ITGA4 subunit is composed of a heavy chain (Tyr 34 - Arg 591, calculated MW 61.1 kDa) and a light chain (Ser 592 - Thr 977, calculated MW 50.4 kDa). Consequently ITGA4 migrates as 60-75 kDa, and ITGB1 as 110-140 kDa when calibrated against Star Ribbon Pre-stained Protein Marker respectively under non-reducing (NR) condition due to cleavage and glycosylation.
內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
純度（Purity）
>90% as determined by SDS-PAGE.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重構方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介紹
整合素是一類跨膜蛋白，可介導相鄰細胞間黏附分子和/或細胞外基質(zhì)（ECM）的相互作用。其在多種生物學過程中發(fā)揮重要作用，包括發(fā)育和傷口愈合過程中的細胞遷移、細胞分化以及細胞凋亡。整合素的活性還能調(diào)節(jié)腫瘤細胞的轉(zhuǎn)移和侵襲潛能。整合素α4β1（Alpha-4/beta-1）是纖連蛋白的受體，同時也可作為VCAM1的受體并識別VCAM1中的Q-I-D-S序列。
關鍵字： 整合素α4β1;Integrin α4β1蛋白;α4β1重組蛋白;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領域提供關鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學等。