Human Integrin alpha 4 beta 7 (ITGA4&ITGB7) Heterodimer Protein, His Tag&Tag Free (MALS & SPR verified)
分子別名（Synonym）
Integrin alpha 4 beta 7,ITGA4&ITGB7
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (IT7-H52W4) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Glu 20 - His 723 (ITGB7) (Accession # P13612-1 (ITGA4) & P26010-1 (ITGB7)).
Predicted N-terminus: Tyr 34 (ITGA4) & Glu 20 (ITGB7)
蛋白結(jié)構(gòu)（Molecular Characterization）
Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA4 and ITGB7, has a calculated MW of 111.4 kDa (ITGA4) & 83.7 kDa (ITGB7). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB7 contains no tag but a basic tail at the C-terminus. The protein migrates as 130-150 kDa (ITGA4) and 65 kDa, 70 kDa, 100-120 kDa (ITGB7) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
純度（Purity）
>90% as determined by SDS-PAGE.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲(chǔ)（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介紹
整合素α4β7是一種黏附受體，屬于整合素家族，與α4β1（VLA4）及E-鈣黏蛋白受體αEβ7共享亞基。該受體對(duì)淋巴細(xì)胞歸巢至腸道黏膜至關(guān)重要，其表達(dá)在派爾集合淋巴結(jié)（Peyer’s patches）或腸系膜淋巴結(jié)的T細(xì)胞活化過(guò)程中被誘導(dǎo)產(chǎn)生，且依賴(lài)于局部樹(shù)突細(xì)胞與基質(zhì)細(xì)胞分泌的視黃酸等信號(hào)分子。
HIV-1包膜蛋白gp120可與活化狀態(tài)的整合素α4β7結(jié)合，此相互作用可能（但也可能無(wú)關(guān)）解釋HIV-1病毒在腸道相關(guān)淋巴組織（GALT）中的富集現(xiàn)象。此外，整合素α4β7還可能參與急性腸道移植物抗宿主?。?/span>GVHD）中的淋巴細(xì)胞遷移過(guò)程。
關(guān)鍵字： Integrin α4β7;整合素α4β7;Integrin α4β7蛋白;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國(guó)生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺(tái)型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國(guó)、美國(guó)、瑞士等12個(gè)城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計(jì)服務(wù)客戶(hù)超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長(zhǎng)期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。