產(chǎn)品概述
| 產(chǎn)品名稱(Product Name) | R40037 |
| 描述(Description) | Rabbit Monoclonal antibody |
| 宿主(Host) | Rabbit |
| 應(yīng)用(Application) | WB,IHC-F,IHC-P,ICC/IF |
| 種屬反應(yīng)性(Reactivity) | Human, Mouse, Rat |
產(chǎn)品性能
| 偶聯(lián)物(Conjugation) | Unconjugated |
| 修飾(Modification) | Monomethylated |
| 同種型(Isotype) | IgG |
| 克?。–lonality) | Monoclonal Antibody |
| 形式(Form) | Liquid |
| 存放說明(Storage) | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 儲(chǔ)存溶液(Buffer) | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. |
| 純化方式(Purification) | Affinity Purified |
免疫原
| 基因名(Gene Name) | H4C1 |
| 別名(Alternative Names) | H4K20me; H4; H4/n; H4F2; H4FN; FO108; HIST2H4 |
| 基因ID(Gene ID) | |
| 蛋白ID(SwissProt ID) | P62805 |
產(chǎn)品應(yīng)用
| 稀釋比(Dilution Ratio) | WB 1:500-1:1000 IHC 1:50-1:100 IF: 1:50-1:200 |
| 蛋白分子量(Molecular Weight) | Calculated MW:11 kDa;Observed MW: 11 kDa |
研究背景
Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, tri-methylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors.
研究領(lǐng)域
關(guān)鍵字: H4C1;MonoMethyl-Histone;H4;(Lys20);Rabbit;Monoclonal;Antibody;一抗
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