產(chǎn)品概述
| 產(chǎn)品名稱（Product Name） | KMT6 / EZH2 (6S19) Rabbit Monoclonal Antibody |
| 描述（Description） | Rabbit Monoclonal Antibody |
| 宿主（Host） | Rabbit |
| 應(yīng)用（Application） | WB,IHC-P,ICC/IF,FC,IF-P |
| 種屬反應(yīng)性（Reactivity） | Human,Mouse,Rat |
產(chǎn)品性能
| 偶聯(lián)物（Conjugation） | Unconjugated |
| 修飾（Modification） | Unmodified |
| 同種型（Isotype） | IgG |
| 克?。–lonality） | Monoclonal |
| 形式（Form） | Liquid |
| 存放說明（Storage） | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 儲存溶液（Buffer） | Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA. |
| 純化方式（Purification） | Affinity purification |
免疫原
| 基因名（Gene Name） | EZH2 |
| 別名（Alternative Names） | EZH 2; EZH1; EZH2; EZH2b; KMT 6; KMT6; KMT6A; |
| 基因ID（Gene ID） | 2146 |
| 蛋白ID（SwissProt ID） | Q15910 |
產(chǎn)品應(yīng)用
| 稀釋比（Dilution Ratio） | WB 1:1000, IHC-P/IF-P 1:50, ICC/IF 1:50, FCM 1:20 |
| 蛋白分子量（Molecular Weight） | 85kDa |
研究背景
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2 (PubMed:22323599, PubMed:30923826). Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
研究領(lǐng)域
關(guān)鍵字： EZH2 ;KMT6;/;EZH2;(6S19);Rabbit;Monoclonal;Antibody;一抗
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