產(chǎn)品概述
| 產(chǎn)品名稱（Product Name） | PARK7 (9L2) Rabbit Monoclonal Antibody |
| 描述（Description） | Rabbit Monoclonal Antibody |
| 宿主（Host） | Rabbit |
| 應(yīng)用（Application） | WB,IHC-P,ICC/IF,FC,IP,IF-P |
| 種屬反應(yīng)性（Reactivity） | Human,Mouse,Rat |
產(chǎn)品性能
| 偶聯(lián)物（Conjugation） | Unconjugated |
| 修飾（Modification） | Unmodified |
| 同種型（Isotype） | IgG |
| 克?。–lonality） | Monoclonal |
| 形式（Form） | Liquid |
| 存放說明（Storage） | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 儲存溶液（Buffer） | Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA. |
| 純化方式（Purification） | Affinity purification |
免疫原
| 基因名（Gene Name） | PARK7 |
| 別名（Alternative Names） | Protein DJ-1; SP22; Protein DJ-1; Oncogene DJ1; Parkinson disease protein 7; PARK7; |
| 基因ID（Gene ID） | 11315 |
| 蛋白ID（SwissProt ID） | Q99497 |
產(chǎn)品應(yīng)用
| 稀釋比（Dilution Ratio） | WB 1:1000-1:5000, IHC-P/IF-P 1:200-1:1000, ICC/IF 1:50-1:500, FCM 1:20-1:50, IP 1:20 |
| 蛋白分子量（Molecular Weight） | 20kDa |
研究背景
Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress. Multifunctional protein with controversial molecular function which plays an important role in cell protection against oxidative stress and cell death acting as oxidative stress sensor and redox- sensitive chaperone and protease (PubMed:17015834, PubMed:20304780, PubMed:18711745, PubMed:12796482, PubMed:19229105, PubMed:25416785, PubMed:26995087, PubMed:28993701). It is involved in neuroprotective mechanisms like the stabilization of NFE2L2 and PINK1 proteins, male fertility as a positive regulator of androgen signaling pathway as well as cell growth and transformation through, for instance, the modulation of NF-kappa-B signaling pathway (PubMed:12612053, PubMed:15502874, PubMed:14749723, PubMed:17015834, PubMed:21097510, PubMed:18711745). Has been described as a protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals (PubMed:25416785, PubMed:28596309). But this function is rebuted by other works (PubMed:27903648, PubMed:31653696). As a protein deglycase, repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) that cause irreversible damage (PubMed:25416785, PubMed:28013050, PubMed:26995087). Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair (PubMed:28596309). Protects histones from adduction by methylglyoxal, controls the levels of methylglyoxal- derived argininine modifications on chromatin (PubMed:30150385). Able to remove the glycations and restore histone 3, histone glycation disrupts both local and global chromatin architecture by altering histone-DNA interactions as well as histone acetylation and ubiquitination levels (PubMed:30150385, PubMed:30894531). Displays a very low glyoxalase activity that may reflect its deglycase activity (PubMed:22523093, PubMed:31653696, PubMed:28993701). Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death (PubMed:16390825). Required for correct mitochondrial morphology and function as well as for autophagy of dysfunctional mitochondria (PubMed:19229105, PubMed:16632486). Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking (PubMed:18711745). Regulates astrocyte inflammatory responses, may modulate lipid rafts-dependent endocytosis in astrocytes and neuronal cells (PubMed:23847046). In pancreatic islets, involved in the maintenance of mitochondrial reactive oxygen species (ROS) levels and glucose homeostasis in an age- and diet dependent manner. Protects pancreatic beta cells from cell death induced by inflammatory and cytotoxic setting (By similarity). Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress (PubMed:18626009). Metal-binding protein able to bind copper as well as toxic mercury ions, enhances the cell protection mechanism against induced metal toxicity (PubMed:23792957). In macrophages, interacts with the NADPH oxidase subunit NCF1 to direct NADPH oxidase-dependent ROS production, and protects against sepsis (By similarity).
研究領(lǐng)域
關(guān)鍵字： PARK7 ;PARK7;(9L2);Rabbit;Monoclonal;Antibody;一抗
武漢恩璣生命科技有限公司（EnkiLife）是一家深耕生命科學(xué)，專注細(xì)胞生物學(xué)和免疫學(xué)科研試劑的研發(fā)、生產(chǎn)與銷售的生物技術(shù)企業(yè)，在全球100多個(gè)國家和地區(qū)開展業(yè)務(wù)，致力于為科研工作者提供高質(zhì)量的產(chǎn)品和卓越的客戶服務(wù)，推動生命科學(xué)的發(fā)展。
EnkiLife的產(chǎn)品線涵蓋細(xì)胞系、原代細(xì)胞、細(xì)胞培養(yǎng)基、血清、細(xì)胞檢測試劑盒、重組靶點(diǎn)蛋白、細(xì)胞因子、重組抗體、ELISA試劑盒、生化試劑盒等，并提供技術(shù)服務(wù)與定制開發(fā)，覆蓋了生命科學(xué)研究的各大關(guān)鍵領(lǐng)域，包括細(xì)胞生物學(xué)、癌癥、免疫學(xué)、神經(jīng)科學(xué)、心血管疾病、干細(xì)胞、表觀遺傳學(xué)、內(nèi)分泌、蛋白質(zhì)組學(xué)、代謝組學(xué)等，全方位滿足您的實(shí)驗(yàn)需求，讓您享受科研的樂趣！
公司現(xiàn)已建立四大技術(shù)平臺：
EnCyto?細(xì)胞培養(yǎng)及檢測平臺：擁有細(xì)胞系庫（500+）、原代細(xì)胞庫（500+）、基礎(chǔ)培養(yǎng)基和完全培養(yǎng)基（1200+）
EnkiPro?重組蛋白平臺：現(xiàn)貨產(chǎn)品2000+，可提供定制化表達(dá)服務(wù)
EnAb?重組抗體平臺：重組兔單抗（3000+），可提供定制化和標(biāo)記服務(wù)
EnKits?試劑盒開發(fā)平臺：可提供優(yōu)質(zhì)的ELISA試劑盒、配套試劑、抗體對、生化試劑盒等相關(guān)產(chǎn)品
EnkiLife在生產(chǎn)管理方面引入ISO9001質(zhì)量管理體系和信息化、自動化的管理工具，擁有高效穩(wěn)定的交付能力，與全球知名品牌建立了緊密的合作。
EnkiLife始終堅(jiān)持以技術(shù)創(chuàng)新為驅(qū)動，以匠心鑄就品質(zhì)，以品質(zhì)服務(wù)客戶。
我們期待與更多的全球科研工作者和企業(yè)攜手合作，共同推動生命科學(xué)領(lǐng)域的進(jìn)步與發(fā)展。