產(chǎn)品概述
Phospho-Artemis (Ser516) Rabbit Polyclonal Antibody
Rabbit Polyclonal Antibody
Rabbit
WB,IHC-P,ELISA
Human,Mouse
產(chǎn)品性能
Unconjugated
Phosphorylated
IgG
Polyclonal Antibody
Liquid
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Affinity Chromatography
免疫原
DCLRE1C
SCIDA; SNM1C; A-SCID; RS-SCID; DCLREC1C
64421
Q96SD1
產(chǎn)品應(yīng)用
WB: 1:500-1:1000 IHC: 1:50-1:100 ELISA: 1:10000
Calculated MW: 78 kDa; Observed MW: 78 kDa
研究背景
Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.
研究領(lǐng)域
Epigenetics and Nuclear Signaling
武漢恩璣生命科技有限公司
聯(lián)系商家時(shí)請(qǐng)?zhí)峒癱hemicalbook,有助于交易順利完成!
在線客服