Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag (MALS verified)
分子別名（Synonym）
IL12, p70, Interleukin-12
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag (IL2-H4210) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (IL-12B) & Arg 23 - Ser 219 (IL-12A) (Accession # P29460-1 (IL12B) & P29459-1 (IL12A)).
Predicted N-terminus: Ile 23 (IL12B) & Arg 23 (IL12A)
蛋白結(jié)構(gòu)（Molecular Characterization）
Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of IL-12B and IL-12A, has a calculated MW of 36.6 kDa (IL-12B) and 23.8 kDa (IL-12A). Subunit IL-12B is fused with polyhistidine tag at the C-terminus and subunit IL-12A is fused with flag tag at the C-terminus. The protein migrates as 30-38 kDa (IL12B) and 40-50 kDa (IL12A) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
純度（Purity）
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲(chǔ)（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介紹
白細(xì)胞介素12（IL-12）又稱p70，是一種由樹(shù)突狀細(xì)胞、巨噬細(xì)胞和人B淋巴母細(xì)胞（NC-37）在抗原刺激下自然產(chǎn)生的白細(xì)胞介素。IL-12是一種異二聚體細(xì)胞因子，由IL-12A（p35）和IL-12B（p40）兩個(gè)亞基組成。該細(xì)胞因子參與初始T細(xì)胞向Th1細(xì)胞的分化過(guò)程，被稱為T細(xì)胞刺激因子，能促進(jìn)T細(xì)胞生長(zhǎng)并增強(qiáng)其功能。它可刺激T細(xì)胞和自然殺傷（NK）細(xì)胞產(chǎn)生γ-干擾素（IFN-γ）和腫瘤壞死因子α（TNF-α），并能降低IL-4對(duì)IFN-γ的抑制作用。IL-12在自然殺傷細(xì)胞和T淋巴細(xì)胞的生理活動(dòng)中具有重要作用，同時(shí)具備抗血管生成活性，即能夠阻斷新生血管的形成。
關(guān)鍵字： IL-12B&IL-12A;IL-12B蛋白;IL-12A重組蛋白;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國(guó)生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺(tái)型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國(guó)、美國(guó)、瑞士等12個(gè)城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計(jì)服務(wù)客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長(zhǎng)期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。