Biotinylated Human Carbonic Anhydrase IX / CA9 (38-414) Protein, His,Avitag? (MALS verified)

分子別名（Synonym）

CAIX, CA9, CA-IX, G250, MN, P54, 58N, pMW1

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Biotinylated Human Carbonic Anhydrase IX (38-414), His,Avitag (CA9-H82E3) is expressed from human 293 cells (HEK293). It contains AA Gln 38 - Asp 414 (Accession # Q16790-1).

Predicted N-terminus: Gln 38

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag?).

The protein has a calculated MW of 44.5 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

The protein is designed as a dimer.

標(biāo)記（Labeling）

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標(biāo)記度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method / rFC method.

純度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in 20 mM MES, 100 mM NaCl, pH6.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介紹

碳酸酐酶（CAs）是一個(gè)龐大的鋅金屬酶家族。這類酶能催化二氧化碳與水快速轉(zhuǎn)化為碳酸氫根和質(zhì)子（或逆向反應(yīng)），該可逆反應(yīng)在無(wú)催化劑條件下進(jìn)行極為緩慢。在動(dòng)物體內(nèi)，碳酸酐酶的功能之一是通過(guò)調(diào)節(jié)二氧化碳與碳酸氫根的相互轉(zhuǎn)化，維持血液及其他組織的酸堿平衡，并協(xié)助二氧化碳從組織中轉(zhuǎn)運(yùn)。大多數(shù)碳酸酐酶的活性中心含有鋅離子，目前已知至少存在五個(gè)不同的CA家族（α、β、γ、δ和ε）。

碳酸酐酶9（CA9/CAIX）又稱膜抗原MN、腎細(xì)胞癌相關(guān)抗原G250，屬于α-碳酸酐酶家族。CA9/CAIX在pH 6.49條件下具有最佳活性，可催化二氧化碳的可逆水合反應(yīng)。CA IX參與細(xì)胞內(nèi)pH調(diào)節(jié)，可能涉及細(xì)胞增殖與轉(zhuǎn)化過(guò)程的調(diào)控。CA-IX被視為宮頸上皮內(nèi)瘤變的新型特異性生物標(biāo)志物。