Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein, His,Avitag?&Tag Free (MALS verified)

分子別名（Synonym）

Integrin alpha V beta 8,ITGAV&ITGB8

表達區(qū)間及表達系統(tǒng)（Source）

Biotinylated Human ITGAV&ITGB8 Heterodimer Protein, His,Avitag&Tag Free (IT8-H82W5) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Glu 43 - Arg 684 (ITGB8) (Accession # NP_002201.1 (ITGAV) & NP_002205.1 (ITGB8)).

Predicted N-terminus: Phe 31 (ITGAV) & Glu 43 (ITGB8)

蛋白結(jié)構(gòu)（Molecular Characterization）

Biotinylated Human ITGAV&ITGB8 Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 114.7 kDa (ITGAV) and 76.5 kDa (ITGB8). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag?) and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 135 kDa (ITGAV) and 80 kDa (ITGB8) respectively due to glycosylation.

標(biāo)記（Labeling）

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標(biāo)記度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介紹

整合素αVβ8（ITGAV與ITGB8或ITGAVB8）表達于卵黃囊、胎盤、腦組織中的血管周圍星形膠質(zhì)細(xì)胞、施萬細(xì)胞、腎小球系膜細(xì)胞及肺上皮細(xì)胞。與其他αV整合素不同，ITGAVB8似乎不呈現(xiàn)不同的激活狀態(tài)，其胞質(zhì)尾部不與細(xì)胞骨架連接。該整合素可結(jié)合含RGD基序的配體，包括玻連蛋白、纖維蛋白以及潛伏TGF-β復(fù)合物中的潛伏相關(guān)肽（LAP）。αVβ8與LAP的高親和力結(jié)合允許MT1-MMP對其進行蛋白水解切割，從而釋放活性TGF-β。此作用機制不同于另一種αV整合素——αVβ6，后者通過非蛋白水解機制從潛伏狀態(tài)激活TGF-β。TGF-β激活的下游效應(yīng)包括調(diào)控細(xì)胞生長及相關(guān)血管化過程。