Biotinylated Human CD2 / SRBC Protein, His,Avitag? (MALS verified)

分子別名（Synonym）

CD2,SRBC,LFA-2,T11

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Biotinylated Human CD2, His,Avitag (CD2-H82E6) is expressed from human 293 cells (HEK293). It contains AA Lys 25 - Asp 209 (Accession # AAH33583).

Predicted N-terminus: Lys 25

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag?).

The protein has a calculated MW of 24.8 kDa. The protein migrates as 35-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

標(biāo)記（Labeling）

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標(biāo)記度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介紹

T細(xì)胞表面抗原CD2（亦稱紅細(xì)胞受體、LFA-2、LFA-3受體、玫瑰花結(jié)受體、T細(xì)胞表面抗原T11/Leu-5及SRBC）是一種表達(dá)于T細(xì)胞和自然殺傷（NK）細(xì)胞表面的I型單次跨膜蛋白。該蛋白屬于免疫球蛋白超家族成員，包含1個(gè)免疫球蛋白樣C2型結(jié)構(gòu)域和1個(gè)免疫球蛋白樣V型結(jié)構(gòu)域。CD2/SRBC可與其他細(xì)胞表面表達(dá)的黏附分子相互作用，如人類淋巴細(xì)胞功能相關(guān)抗原-3（LFA-3/CD58）或嚙齒類動(dòng)物的CD48。除介導(dǎo)細(xì)胞黏附外，CD2還作為T細(xì)胞和NK細(xì)胞的共刺激分子發(fā)揮作用。作為T細(xì)胞與NK細(xì)胞的特異性標(biāo)志物，CD2可通過免疫組織化學(xué)技術(shù)用于組織切片中該類細(xì)胞的鑒定。