Human TNFR2 / CD120b / TNFRSF1B Protein, His Tag (MALS verified)

分子別名（Synonym）

TNFRSF1B,CD120b,TBPII,TNF-R-II,TNF-R75,TNFBR,TNFR1B,TNFR2,TNFR80,p75,p75TNFR

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human TNFR2, His Tag (TN2-H5227) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Asp 257 (Accession # AAA36755).

Predicted N-terminus: Leu 23

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 26.0 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介紹

腫瘤壞死因子受體I（亦稱p60/p55 TNFR）與腫瘤壞死因子受體II（亦稱p75/p80 TNFR）是兩種不同的I型跨膜糖蛋白，能以高親和力結(jié)合腫瘤壞死因子。二者均屬腫瘤壞死因子受體超家族典型成員，分別被命名為TNFRSF1A與TNFRSF1B。人TNF RII的cDNA編碼一個(gè)包含461個(gè)氨基酸殘基的前體蛋白，其結(jié)構(gòu)包括22個(gè)氨基酸的推定信號(hào)肽、235個(gè)氨基酸的胞外結(jié)構(gòu)域、20個(gè)氨基酸的跨膜結(jié)構(gòu)域以及174個(gè)氨基酸的胞內(nèi)結(jié)構(gòu)域。該受體在胎兒腦組織中表達(dá)，其天然可溶性形式（sTNFRII）可通過競(jìng)爭(zhēng)性結(jié)合TNFα阻斷其與細(xì)胞表面受體的結(jié)合，從而抑制TNFα的生物學(xué)效應(yīng)。在類風(fēng)濕關(guān)節(jié)炎多發(fā)家族的病例研究中發(fā)現(xiàn)，TNFRII在軟骨-血管翳交界處顯著表達(dá)并發(fā)揮重要作用。此外，高水平血漿sTNFRII與冠心病發(fā)病風(fēng)險(xiǎn)增加顯著相關(guān)，且這種關(guān)聯(lián)獨(dú)立于傳統(tǒng)心血管危險(xiǎn)因素；該指標(biāo)還可用于監(jiān)測(cè)結(jié)節(jié)病的炎癥活動(dòng)度。