Human CD73 / NT5E Protein, His Tag (HPLC-verified) (active enzyme)

分子別名（Synonym）

CD73, NT5E, 5'-Nucleotidase, 5'-NT, NT5, NTE

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human CD73 Protein, His Tag (CD3-H52H7) is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549 (Accession # P21589-1).

Predicted N-terminus: Trp 27

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 59.9 kDa. The protein migrates as 65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

The protein is designed as a dimer.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

純度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 24 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景介紹

5‘-核苷酸酶（5’-NT）亦稱外切-5‘-核苷酸酶或CD73，是由NT5E基因編碼的一種酶。該酶主要催化腺苷一磷酸（AMP）向腺苷的轉(zhuǎn)化。外切-5‘-核苷酸酶（5’-核糖核苷酸磷酸水解酶）在中性pH條件下可催化嘌呤5‘-單核苷酸向核苷的轉(zhuǎn)化，其最適底物為AMP。細(xì)胞質(zhì)和溶酶體中還存在其他形式的5‘-核苷酸酶，這些同工酶可通過底物親和力、二價(jià)鎂離子需求、ATP激活特性及無機(jī)磷酸抑制效應(yīng)與外切-NT5相區(qū)分。罕見等位基因變異與成人型關(guān)節(jié)動(dòng)脈鈣化綜合征（CALJA）相關(guān)，該病癥累及髂動(dòng)脈、股動(dòng)脈及脛動(dòng)脈，導(dǎo)致下肢及手足關(guān)節(jié)血液循環(huán)障礙并引發(fā)疼痛。