Unconjugated Human TPBG / 5T4 Protein, His,Avitag?

分子別名（Synonym）

TPBG,5T4,M6P1,5T4AG,WAIF1,5T4 oncofetal antigen,Trophoblast glycoprotein,5T4 oncofetal trophoblast glycoprotein

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Unconjugated Human TPBG, His,Avitag (TPG-H52E5) is expressed from human 293 cells (HEK293). It contains AA Ser 32 - Ser 355 (Accession # Q13641-1).

Predicted N-terminus: Ser 32

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag?).

The protein has a calculated MW of 39.0 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

應(yīng)用說(shuō)明（Application）

TPG-H52E5 is the unconjugated version of TPG-H82Eb (Biotinylated Human TPBG, His,Avitag). It can be utilized for assay development or customized biotinylation according to the experimental designs.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method / rFC method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介紹

滋養(yǎng)層細(xì)胞糖蛋白（TPBG），亦稱(chēng)5T4，是多個(gè)臨床研發(fā)階段抗癌藥物的治療靶點(diǎn)，這主要?dú)w因于其在腫瘤組織中高表達(dá)、在正常成人組織中低表達(dá)的特性。該基因編碼一種富含亮氨酸的跨膜糖蛋白，可能參與細(xì)胞粘附過(guò)程。TPBG在胚胎發(fā)育早期（最早9周）即表達(dá)于所有類(lèi)型的滋養(yǎng)層細(xì)胞中。