Biotinylated Human IL-2 R alpha / CD25 Protein, His,Avitag?, premium grade

分子別名（Synonym）

IL2RA,CD25,p55,IL2-RA,IL-2-RA

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Biotinylated Human IL-2 R alpha, His,Avitag, premium grade (ILA-H82E6) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # P01589-1).

Predicted N-terminus: Glu 22

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蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag?).

The protein has a calculated MW of 25.4 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

標(biāo)記（Labeling）

Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標(biāo)記度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method / rFC method.

無菌（Sterility）

Negative

支原體（Mycoplasma）

Negative

純度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介紹

白細(xì)胞介素-2受體α亞基（IL2RA）又稱IL-2Rα、CD25抗原，是一種I型跨膜糖蛋白。該蛋白表達(dá)于活化T細(xì)胞與調(diào)節(jié)性T細(xì)胞表面，可單獨(dú)以低親和力與IL-2結(jié)合。當(dāng)IL2RA與IL-2受體β鏈、γ鏈通過非共價(jià)鍵形成配體誘導(dǎo)的高親和力異源三聚體受體復(fù)合物后，能夠啟動(dòng)MAPK、JAK/STAT等細(xì)胞內(nèi)信號通路。在樹突狀細(xì)胞中，CD25雖曾被視作活化標(biāo)志物，但值得注意的是，盡管人類及小鼠樹突狀細(xì)胞均可表達(dá)CD25，卻缺乏IL-2信號傳導(dǎo)所必需的β鏈，因此無法執(zhí)行IL-2信號傳導(dǎo)功能。