Human IL-2 R alpha / CD25 Protein, Fc Tag (MALS verified)

分子別名（Synonym）

IL2RA, CD25, p55, IL2-RA, IL-2-RA

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human IL-2 R alpha Protein, Fc Tag (ILA-H5251) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # NP_000408).

Predicted N-terminus: Glu 22

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 48.4 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

純度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介紹

白細(xì)胞介素-2受體α亞基（IL2RA）又稱IL-2R α鏈、IL-2-RA、IL2-RA、TAC抗原、p55、CD25抗原，是一種I型跨膜糖蛋白。該蛋白在活化T細(xì)胞與調(diào)節(jié)T細(xì)胞表面表達(dá)，具備獨(dú)立低親和力結(jié)合IL-2的能力。當(dāng)IL2RA通過非共價鍵與IL-2受體β鏈、γ鏈組裝后，可形成配體誘導(dǎo)的高親和力異源三聚體受體復(fù)合物，進(jìn)而啟動MAPK、JAK/STAT等細(xì)胞內(nèi)信號通路。在樹突狀細(xì)胞中，CD25長期被視為活化標(biāo)志物——但需注意，盡管小鼠與人類樹突狀細(xì)胞均能表達(dá)CD25，卻均不表達(dá)IL-2信號傳導(dǎo)所必需的IL-2受體β鏈。