Human IL-3 R alpha / CD123 Protein, His Tag (MALS & SPR verified)

分子別名（Synonym）

IL3R,IL3RA,IL-3Ra,IL-3R-alpha,IL3RAY,IL3RX,IL3RY,CD123 antigen,CD123,hIL3Ra,hIL-3Ra,MGC34174,IL-3 R alpha

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human IL-3 R alpha, His Tag (ILA-H52H6) is expressed from human 293 cells (HEK293). It contains AA Thr 19 - Arg 305  (Accession # P26951-1).

Predicted N-terminus: Thr 19

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 35.0 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介紹

白細(xì)胞介素3受體α鏈（低親和力）（IL3RA）亦稱(chēng)為分化簇123（CD123），是造血素受體超家族中一種70kD的糖蛋白。該蛋白通過(guò)與IL-5及粒細(xì)胞-巨噬細(xì)胞集落刺激因子（GM-CSF）受體共有的β亞基結(jié)合，形成IL-3的高親和力受體。由于在急性髓系白血?。?/span>AML）中白細(xì)胞介素-3受體α鏈（CD123）的表達(dá)水平顯著高于正常造血干細(xì)胞，該蛋白已被視為潛在的免疫治療靶點(diǎn)。