Biotinylated Human SCF / KITLG Protein, Avitag?,His Tag
分子別名(Synonym)
KITLG,FPH2,KL-1,Kitl,MGF,SCF,SF,SHEP7,KL
表達區(qū)間及表達系統(tǒng)(Source)
Biotinylated Human SCF, Avitag,His Tag (SCF-H82E1) is expressed from human 293 cells (HEK293). It contains AA Glu 26 - Ala 190 (Accession # AAH69797).
Predicted N-terminus: Glu 26
蛋白結(jié)構(gòu)(Molecular Characterization)

This protein carries an Avi tag (Avitag?) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 21.7 kDa. The protein migrates as 26-38 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
標記(Labeling)
Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白標記度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
純度(Purity)
>95% as determined by SDS-PAGE.
制劑(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

背景介紹
干細胞因子(SCF)亦稱kit配體、KL、Steel因子、KITLG、FPH2、KL-1、Kitl、MGF、SF或SHEP7,是一種能與c-Kit受體(CD117)結(jié)合的細胞因子。該因子既可作為跨膜蛋白存在,也可表現(xiàn)為可溶性蛋白形式。這種細胞因子在造血作用(血細胞形成)、精子發(fā)生和黑色素生成過程中發(fā)揮重要功能。其可溶性及跨膜形式由同一RNA轉(zhuǎn)錄本通過可變剪接形成。
成纖維細胞與內(nèi)皮細胞可同時產(chǎn)生可溶性及跨膜型SCF??扇苄?/span>SCF分子量為18.5 kDa,并以二聚體形式存在。在胚胎發(fā)育過程中,SCF對造血功能具有關(guān)鍵調(diào)控作用。胎兒肝臟、骨髓等造血部位均表達SCF。在發(fā)育過程中,SCF還通過調(diào)控黑色素細胞(負責產(chǎn)生黑色素及控制色素沉著的細胞)的定位發(fā)揮重要作用。
SCF參與調(diào)控骨髓干細胞微環(huán)境中的造血干細胞(HSCs)。在體外培養(yǎng)體系中,SCF可與其他細胞因子聯(lián)用于造血干細胞及造血祖細胞的培養(yǎng)。這些細胞的體外擴增技術(shù)將推動骨髓移植領(lǐng)域的發(fā)展——通過移植造血干細胞至患者體內(nèi),重建其造血功能。
關(guān)鍵字: SCF;SCF蛋白;SCF重組蛋白;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group(股票代碼:301080)是成立于2010年的跨國生物科技公司,是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務遍布全球,橫跨亞洲、北美洲、歐洲,在中國、美國、瑞士等12個城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務客戶超6000家,與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學等。