產品概述
| 產品名稱(Product Name) | CaMKIα (phospho Thr177) Rabbit Polyclonal Antibody |
| 描述(Description) | Rabbit Polyclonal Antibody |
| 宿主(Host) | Rabbit |
| 應用(Application) | WB,IHC-P,IF-P,IF-F,ICC/IF,ELISA |
| 種屬反應性(Reactivity) | Human,Mouse,Rat |
產品性能
| 偶聯(lián)物(Conjugation) | Unconjugated |
| 修飾(Modification) | Phospho Antibody |
| 同種型(Isotype) | IgG |
| 克?。–lonality) | Polyclonal |
| 形式(Form) | Liquid |
| 存放說明(Storage) | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 儲存溶液(Buffer) | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N. |
| 純化方式(Purification) | Affinity purification |
免疫原
| 基因名(Gene Name) | CAMK1 |
| 別名(Alternative Names) | CAMK1; Calcium/calmodulin-dependent protein kinase type 1; CaM kinase I; CaM-KI; CaM kinase I alpha; CaMKI-alpha |
| 基因ID(Gene ID) | 8536 |
| 蛋白ID(SwissProt ID) | Q14012 |
產品應用
| 稀釋比(Dilution Ratio) | WB 1:500-1:2000, IHC-P 1:100-1:300, IF-P/IF-F/ICC/IF 1:200-1:1000, ELISA 1:5000.Not yet tested in other applications. |
| 蛋白分子量(Molecular Weight) | 41kDa |
研究背景
Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008],catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.,enzyme regulation:Activated by Ca(2+)/calmodulin. Binding of calmodulin results in a conformational change that generates functional binding sites for both, substrate and ATP, and thus releaves intrasteric autoinhibition. Must be phosphorylated to be maximally active. Phosphorylated by CAMKK1 or CAMKK2.,function:Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes like transcriptional regulation, hormone production, translational regulation, regulation of actin filament organization and neurite outgrowth. Involved in calcium-dependent activation of the ERK pathway (By similarity). Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CTFR, MYL9, SYN1/synapsin I and SYNII/synapsin II.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Predominantly cytoplasmic.,subunit:Monomer. Interacts with XPO1.,tissue specificity:Ubiquitous.,
研究領域
關鍵字: CAMK1;CaMKIα;(phospho;Thr177);Rabbit;Polyclonal;Antibody;一抗
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