產(chǎn)品概述
| 產(chǎn)品名稱（Product Name） | ZAP-70 Rabbit Polyclonal Antibody |
| 描述（Description） | Rabbit Polyclonal Antibody |
| 宿主（Host） | Rabbit |
| 應(yīng)用（Application） | WB,IHC-P,IF-P,IF-F,ICC/IF,ELISA |
| 種屬反應(yīng)性（Reactivity） | Human,Mouse,Rat |
產(chǎn)品性能
| 偶聯(lián)物（Conjugation） | Unconjugated |
| 修飾（Modification） | Unmodified |
| 同種型（Isotype） | IgG |
| 克?。–lonality） | Polyclonal |
| 形式（Form） | Liquid |
| 存放說(shuō)明（Storage） | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 儲(chǔ)存溶液（Buffer） | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N. |
| 純化方式（Purification） | Affinity purification |
免疫原
| 基因名（Gene Name） | ZAP70 |
| 別名（Alternative Names） | ZAP70; SRK; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase |
| 基因ID（Gene ID） | 7535 |
| 蛋白ID（SwissProt ID） | P43403 |
產(chǎn)品應(yīng)用
| 稀釋比（Dilution Ratio） | WB 1:500-1:2000, IHC-P 1:100-1:300, IF-P/IF-F/ICC/IF 1:200-1:1000, ELISA 1:10000.Not yet tested in other applications. |
| 蛋白分子量（Molecular Weight） | 70kDa |
研究背景
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.,domain:The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.,function:Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.,online information:ZAP70 mutation db,PTM:Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 SH2 domains.,subcellular location:After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic.,subunit:Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB (By similarity). Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6 (By similarity). Interacts with FCRL3.,tissue specificity:Expressed in T- and natural killer cells.,
研究領(lǐng)域
Natural killer cell mediated cytotoxicity;T_Cell_Receptor;Primary immunodeficiency;
關(guān)鍵字： ZAP70;ZAP-70;Rabbit;Polyclonal;Antibody;一抗
武漢恩璣生命科技有限公司（EnkiLife）是一家深耕生命科學(xué)，專注細(xì)胞生物學(xué)和免疫學(xué)科研試劑的研發(fā)、生產(chǎn)與銷售的生物技術(shù)企業(yè)，在全球100多個(gè)國(guó)家和地區(qū)開展業(yè)務(wù)，致力于為科研工作者提供高質(zhì)量的產(chǎn)品和卓越的客戶服務(wù)，推動(dòng)生命科學(xué)的發(fā)展。
EnkiLife的產(chǎn)品線涵蓋細(xì)胞系、原代細(xì)胞、細(xì)胞培養(yǎng)基、血清、細(xì)胞檢測(cè)試劑盒、重組靶點(diǎn)蛋白、細(xì)胞因子、重組抗體、ELISA試劑盒、生化試劑盒等，并提供技術(shù)服務(wù)與定制開發(fā)，覆蓋了生命科學(xué)研究的各大關(guān)鍵領(lǐng)域，包括細(xì)胞生物學(xué)、癌癥、免疫學(xué)、神經(jīng)科學(xué)、心血管疾病、干細(xì)胞、表觀遺傳學(xué)、內(nèi)分泌、蛋白質(zhì)組學(xué)、代謝組學(xué)等，全方位滿足您的實(shí)驗(yàn)需求，讓您享受科研的樂(lè)趣！
公司現(xiàn)已建立四大技術(shù)平臺(tái)：
EnCyto?細(xì)胞培養(yǎng)及檢測(cè)平臺(tái)：擁有細(xì)胞系庫(kù)（500+）、原代細(xì)胞庫(kù)（500+）、基礎(chǔ)培養(yǎng)基和完全培養(yǎng)基（1200+）
EnkiPro?重組蛋白平臺(tái)：現(xiàn)貨產(chǎn)品2000+，可提供定制化表達(dá)服務(wù)
EnAb?重組抗體平臺(tái)：重組兔單抗（3000+），可提供定制化和標(biāo)記服務(wù)
EnKits?試劑盒開發(fā)平臺(tái)：可提供優(yōu)質(zhì)的ELISA試劑盒、配套試劑、抗體對(duì)、生化試劑盒等相關(guān)產(chǎn)品
EnkiLife在生產(chǎn)管理方面引入ISO9001質(zhì)量管理體系和信息化、自動(dòng)化的管理工具，擁有高效穩(wěn)定的交付能力，與全球知名品牌建立了緊密的合作。
EnkiLife始終堅(jiān)持以技術(shù)創(chuàng)新為驅(qū)動(dòng)，以匠心鑄就品質(zhì)，以品質(zhì)服務(wù)客戶。
我們期待與更多的全球科研工作者和企業(yè)攜手合作，共同推動(dòng)生命科學(xué)領(lǐng)域的進(jìn)步與發(fā)展。