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HSC70 抗體
品牌：MedChemExpress (MCE)
貨號：HY-P85704
存儲條件：-20°C保存1年。避免重復(fù)凍結(jié)/解凍循環(huán)。
運(yùn)輸條件：用藍(lán)冰運(yùn)輸。
產(chǎn)品活性：HSC70 Antibody (YA5396) 是一種小鼠來源、無偶聯(lián)標(biāo)記、抗 HSC70 的單克隆抗體。它可用于人、小鼠、大鼠背景下的 IHC-P、WB 實驗。
研究背景：HSC70 是一種分子伴侶，參與多種細(xì)胞過程，包括保護(hù)蛋白質(zhì)組免受應(yīng)激、新合成多肽的折疊和運(yùn)輸、伴侶介導(dǎo)的自噬 (chaperone-mediated autophagy)、錯誤折疊蛋白的激活降解、蛋白質(zhì)復(fù)合物的形成和解離以及抗原呈遞。它在蛋白質(zhì)質(zhì)量控制系統(tǒng)中發(fā)揮關(guān)鍵作用，確保蛋白質(zhì)的正確折疊、錯誤折疊蛋白的重新折疊以及控制蛋白質(zhì)的后續(xù)降解靶向。這一過程通過 ATP 結(jié)合、ATP 水解和 ADP 釋放的循環(huán)實現(xiàn)，并由共伴侶 (co-chaperones) 介導(dǎo)。HSC70 對多肽的親和力受其核苷酸結(jié)合狀態(tài)調(diào)控：ATP 結(jié)合狀態(tài)下對底物蛋白親和力低，而 ATP 水解為 ADP 后構(gòu)象變化會增加親和力。其共伴侶分為三類：J 結(jié)構(gòu)域共伴侶 HSP40s（刺激 HSC70 的 ATPase 活性）、核苷酸交換因子 (NEF) 如 BAG1/2/3（促進(jìn) ADP 到 ATP 的轉(zhuǎn)換從而釋放底物）以及 TPR 結(jié)構(gòu)域伴侶如 HOPX 和 STUB1。此外，HSC70 在線粒體導(dǎo)入中起關(guān)鍵作用，將前蛋白遞送至線粒體導(dǎo)入受體 TOMM70；抑制轉(zhuǎn)錄激活；作為 PRP19-CDC5L 復(fù)合物的組分參與剪接體激活；結(jié)合細(xì)菌脂多糖 (LPS) 介導(dǎo)炎癥反應(yīng)；在伴侶介導(dǎo)的自噬中識別含 -KFERQ 基序的底物并引導(dǎo)至溶酶體降解；與 LAMP2 協(xié)作促進(jìn)胞質(zhì)抗原的 MHC II 類呈遞；參與 ER 相關(guān)降解 (ERAD) 通路；并通過與 DNAJC6 相互作用被招募至網(wǎng)格蛋白包被囊泡，激活 ATPase 活性以脫去網(wǎng)格蛋白涂層 (by similar)。
宿主：Mouse
反應(yīng)物種：Human, Mouse, Rat
推薦稀釋比例：IHC1:50-200; WB: 1:1000-2000 Immunohistochemical analysis of paraffin-embedded mouse colon tissue using HSC70 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using HSC70 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
基因 ID：3312
同用名：Heat shock cognate 71 kDa protein; Heat shock 70 kDa protein 8;
克隆性：Monoclonal
偶聯(lián)：Non-conjugated
修飾：Unmodified
蛋白數(shù)據(jù)庫：P11142
詳情參考：www.medchemexpress.cn/antibody/hsc70-antibody-ya5396.html